Metabolic regulation of pigment formation of Onosma paniculatum cultured cells.

The pigment yields of Onosma paniculatum callus and suspension cultured cells were increased 5.5 and 8.1 fold of that of the control respectively, when 10(-5) M of copper ion was added into the medium. The pigments synthesis was also stimulated greatly when 10(-5) M of L-phenylalanine was added into the medium and the cells were cultured for twenty-one days. The maximum yield of pigments was obtained when 10(-6) M of ascorbic acid was added to the medium.