| Literature DB >> 1342608 |
A M Fontes1, J C de-Almeida, J E Edstrom, M L Paçó-Larson.
Abstract
A recombinant clone carrying a 2-kb fragment was isolated from a mini-library of the B10 DNA puff of Bradysia hygida. This fragment was amplified in the salivary gland during the period of DNA puff formation. Amplification started when DNA puff anlage was formed and continued to increase, reaching a maximum of about 10-fold 28 h later. Northern blot hybridization experiments showed that this 2-kb fragment was complementary to two RNA species of about 1.3 kb and 1.1 kb, which are developmentally regulated in the salivary gland. Maximum amounts of these messages were present when the B10 puff is fully expanded.Entities:
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Year: 1992 PMID： 1342608
Source DB: PubMed Journal: Braz J Med Biol Res ISSN： 0100-879X Impact factor: 2.590