Literature DB >> 1339360

Cloning and characterization of a gene from Bacillus stearothermophilus var. non-diastaticus encoding a glycerol dehydrogenase.

P R Mallinder1, A Pritchard, A Moir.   

Abstract

A 4.1-kb EcoRI fragment which includes the gene (gldA) encoding a glycerol dehydrogenase (G1DH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from Bacillus stearothermophilus var. non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to Escherichia coli glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD) mutants. Sequencing suggests that the gldA gene is likely to be monocistronic and encodes a protein of 39450 Da. The deduced amino acid composition and sequence of G1DH reveals that the protein is extremely similar to a characterized metal-dependent NAD-dependent G1DH from B. stearothermophilus RS93. The enzyme has limited homology to the iron-activated alcohol dehydrogenase of Zymomonas mobilis and the butanol dehydrogenase of Clostridium acetobutylicum.

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Year:  1992        PMID： 1339360     DOI： 10.1016/0378-1119(92)90438-u

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  10 in total

1.  Novel listerial glycerol dehydrogenase- and phosphoenolpyruvate-dependent dihydroxyacetone kinase system connected to the pentose phosphate pathway.

Authors:  Céline Monniot; Arthur Constant Zébré; Francine Moussan Désirée Aké; Josef Deutscher; Eliane Milohanic
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2.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

3.  Biochemical and molecular characterization of the oxidative branch of glycerol utilization by Citrobacter freundii.

Authors:  R Daniel; K Stuertz; G Gottschalk
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

4.  SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases.

Authors:  E V Koonin; K E Rudd
Journal:  Nucleic Acids Res       Date:  1993-11-25       Impact factor: 16.971

5.  Characterization of the ptr6(+) gene in fission yeast: a possible involvement of a transcriptional coactivator TAF in nucleocytoplasmic transport of mRNA.

Authors:  T Shibuya; S Tsuneyoshi; A K Azad; S Urushiyama; Y Ohshima; T Tani
Journal:  Genetics       Date:  1999-07       Impact factor: 4.562

6.  Molecular characterization of the glycerol-oxidative pathway of Clostridium butyricum VPI 1718.

Authors:  Céline Raynaud; Jieun Lee; Patricia Sarçabal; Christian Croux; Isabelle Meynial-Salles; Philippe Soucaille
Journal:  J Bacteriol       Date:  2011-04-08       Impact factor: 3.490

7.  Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase.

Authors:  K P Fong; C B Goh; H M Tan
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

8.  Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.

Authors:  H J Sofia; V Burland; D L Daniels; G Plunkett; F R Blattner
Journal:  Nucleic Acids Res       Date:  1994-07-11       Impact factor: 16.971

9.  Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.

Authors:  F R Blattner; V Burland; G Plunkett; H J Sofia; D L Daniels
Journal:  Nucleic Acids Res       Date:  1993-11-25       Impact factor: 16.971

10.  The presence of 3-hydroxypropionate and 1,3-propanediol suggests an alternative path for conversion of glycerol to Acetyl-CoA.

Authors:  Eunsook S Jin; Min H Lee; Craig R Malloy
Journal:  Metabol Open       Date:  2021-02-26
  10 in total

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