Structure of the Cochliobolus heterostrophus glyceraldehyde-3-phosphate dehydrogenase gene.

A single gene (GPD1) encoding glyceraldehyde-3-phosphate dehydrogenase (GPD) was found in Cochliobolus heterostrophus. Homology with other fungal GPD-encoding genes was substantial at both the nucleotide and amino-acid levels. Positions of four introns found in GPD1 were conserved in the corresponding Aspergillus nidulans gpdA gene (which is known to have three additional introns absent in GPD1). The size (approximately 1300 nucleotides) of the single GPD1 transcript was consistent with the length (1011 bp) of the open reading frame. Several transcription initiation sites were identified, including major ones 45 and 40 bp upstream of the start codon. Conserved regulatory sequences were found in both the 5' and 3' flanking regions of GPD1.