Literature DB >> 1336400

Transcobalamin II--cobalamin binding sites are present on rabbit germ cells.

E Boukhzer1, A Ennya, F Felden, A Gérard, E Nexo, J P Nicolas, H Gérard, J L Guéant.   

Abstract

Specific binding sites for rabbit transcobalamin II have been found on isolated adult rabbit germ cells. Scatchard analysis revealed a single class of binding sites for [57Co]cyanocobalamin-transcobalamin II with an association constant (Ka) of 1.3 x 10(10) M-1 and 700 sites per cell. Binding was reversible, saturable and calcium dependent. Electron microscope radioautography following incubation with iodinated transcobalamin II at 4 degrees C led to a detectable labeling mainly restricted to the plasma membrane.

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Year:  1992        PMID: 1336400     DOI: 10.1016/0167-4889(92)90019-8

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  1 in total

1.  Megalin-mediated endocytosis of transcobalamin-vitamin-B12 complexes suggests a role of the receptor in vitamin-B12 homeostasis.

Authors:  S K Moestrup; H Birn; P B Fischer; C M Petersen; P J Verroust; R B Sim; E I Christensen; E Nexø
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

  1 in total

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