Literature DB >> 1335237

Basal and phorbol-ester-stimulated turnover of phosphatidylcholine in HeLa cells involve different pathways.

C S Hii1, Y S Edwards, A W Murray.   

Abstract

HeLa cells prelabelled with [3H]lysoplatelet-activating factor (lyso-PAF) accumulated phosphatidic acid (PtdOH) when incubated in the presence of either propranolol (an inhibitor of PtdOH phosphohydrolase) or phorbol ester. In the presence of ethanol, phorbol ester but not propranolol stimulated the accumulation of phosphatidylethanol, an index of phospholipase D activity. Incubation of cells with [3H]lyso-PAF led to a rapid accumulation of label in diacylglycerol (DG) followed by a delayed accumulation in PtdOH. It is concluded that propranolol-induced PtdOH accumulation is derived from DG by DG kinase and does not involve phospholipase D.

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Year:  1992        PMID： 1335237      PMCID： PMC1131984          DOI： 10.1042/bj2880983

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  A rapid method of total lipid extraction and purification.

Authors:  E G BLIGH; W J DYER
Journal:  Can J Biochem Physiol       Date:  1959-08

Review 2.  Receptor-coupled phospholipase D: regulation and functional significance.

Authors:  M M Billah; J C Anthes; T J Mullmann
Journal:  Biochem Soc Trans       Date:  1991-04       Impact factor: 5.407

3.  Phosphatidylethanol biosynthesis in ethanol-exposed NG108-15 neuroblastoma X glioma hybrid cells. Evidence for activation of a phospholipase D phosphatidyl transferase activity by protein kinase C.

Authors:  M Liscovitch
Journal:  J Biol Chem       Date:  1989-01-25       Impact factor: 5.157

4.  Insertion of fluorescent phospholipids into the plasma membrane of a mammalian cell.

Authors:  D K Struck; R E Pagano
Journal:  J Biol Chem       Date:  1980-06-10       Impact factor: 5.157

5.  Analysis of the water-soluble products of phosphatidylcholine breakdown by ion-exchange chromatography. Bombesin and TPA (12-O-tetradecanoylphorbol 13-acetate) stimulate choline generation in Swiss 3T3 cells by a common mechanism.

Authors:  S J Cook; M J Wakelam
Journal:  Biochem J       Date:  1989-10-15       Impact factor: 3.857

6.  Gonadotropin-releasing hormone activates phospholipase D in ovarian granulosa cells. Possible role in signal transduction.

Authors:  M Liscovitch; A Amsterdam
Journal:  J Biol Chem       Date:  1989-07-15       Impact factor: 5.157

7.  Tumor promoter stimulation of phosphatidylcholine turnover in HeLa cells.

Authors:  G R Guy; A W Murray
Journal:  Cancer Res       Date:  1982-05       Impact factor: 12.701

8.  Phorbol ester-stimulated hydrolysis of phosphatidylcholine and phosphatidylethanolamine by phospholipase D in HeLa cells. Evidence that the basal turnover of phosphoglycerides does not involve phospholipase D.

Authors:  C S Hii; Y S Edwards; A W Murray
Journal:  J Biol Chem       Date:  1991-10-25       Impact factor: 5.157

9.  Phosphatidylethanol formation via transphosphatidylation by rat brain synaptosomal phospholipase D.

Authors:  M Kobayashi; J N Kanfer
Journal:  J Neurochem       Date:  1987-05       Impact factor: 5.372

10.  Phospholipase D catalyzes phospholipid metabolism in chemotactic peptide-stimulated HL-60 granulocytes.

Authors:  J K Pai; M I Siegel; R W Egan; M M Billah
Journal:  J Biol Chem       Date:  1988-09-05       Impact factor: 5.157
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  2 in total

Review 1.  Phospholipase D: molecular and cell biology of a novel gene family.

Authors:  M Liscovitch; M Czarny; G Fiucci; X Tang
Journal:  Biochem J       Date:  2000-02-01       Impact factor: 3.857

2.  Accumulation of phosphatidylalcohol in cultured cells: use of subcellular fractionation to investigate phospholipase D activity during signal transduction.

Authors:  Y S Edwards; A W Murray
Journal:  Biochem J       Date:  1995-06-01       Impact factor: 3.857
  2 in total

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