Literature DB >> 1334069

Escherichia coli cyclic AMP receptor protein mutants provide evidence for ligand contacts important in activation.

J Moore1, M Kantorow, D Vanderzwaag, K McKenney.   

Abstract

The three-dimensional model of the Escherichia coli cyclic AMP (cAMP) receptor protein (CRP) shows that several amino acids are involved as chemical contacts for binding cAMP. We have constructed and characterized mutants at four of these positions, E72, R82, S83, and R123. The mutations were made in wild-type crp as well as a cAMP-independent crp, crp*. The activities of the mutant proteins were characterized in vivo for their ability to activate the lac operon. These results provide genetic evidence to support that E72 and R82 are essential and S83 and R123 are important in the activation of CRP by cAMP.

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Year:  1992        PMID： 1334069      PMCID： PMC207541          DOI： 10.1128/jb.174.24.8030-8035.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

1.  Analogs of cyclic AMP that elicit the biochemically defined conformational change in catabolite gene activator protein (CAP) but do not stimulate binding to DNA.

Authors:  R H Ebright; S F Le Grice; J P Miller; J S Krakow
Journal:  J Mol Biol       Date:  1985-03-05       Impact factor: 5.469

2.  5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis.

Authors:  J R Sayers; W Schmidt; F Eckstein
Journal:  Nucleic Acids Res       Date:  1988-02-11       Impact factor: 16.971

3.  Structural similarity in the DNA-binding domains of catabolite gene activator and cro repressor proteins.

Authors:  T A Steitz; D H Ohlendorf; D B McKay; W F Anderson; B W Matthews
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

4.  Molecular basis of DNA sequence recognition by the catabolite gene activator protein: detailed inferences from three mutations that alter DNA sequence specificity.

Authors:  R H Ebright; P Cossart; B Gicquel-Sanzey; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

5.  Improved oligonucleotide site-directed mutagenesis using M13 vectors.

Authors:  P Carter; H Bedouelle; G Winter
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

6.  Sites of allosteric shift in the structure of the cyclic AMP receptor protein.

Authors:  S Garges; S Adhya
Journal:  Cell       Date:  1985-07       Impact factor: 41.582

7.  Mutations in the cyclic AMP binding site of the cyclic AMP receptor protein of Escherichia coli.

Authors:  A M Gronenborn; R Sandulache; S Gärtner; G M Clore
Journal:  Biochem J       Date:  1988-08-01       Impact factor: 3.857

8.  Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products.

Authors:  P Reddy; A Peterkofsky; K McKenney
Journal:  Nucleic Acids Res       Date:  1989-12-25       Impact factor: 16.971

9.  Role of glutamic acid-181 in DNA-sequence recognition by the catabolite gene activator protein (CAP) of Escherichia coli: altered DNA-sequence-recognition properties of [Val181]CAP and [Leu181]CAP.

Authors:  R H Ebright; A Kolb; H Buc; T A Kunkel; J S Krakow; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

10.  Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.

Authors:  R H Ebright; P Cossart; B Gicquel-Sanzey; J Beckwith
Journal:  Nature       Date:  1984 Sep 20-26       Impact factor: 49.962
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  12 in total

Review 1.  Nitrogen control in cyanobacteria.

Authors:  A Herrero; A M Muro-Pastor; E Flores
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

2.  Cyclic AMP-dependent osmoregulation of crp gene expression in Escherichia coli.

Authors:  Carlos Balsalobre; Jörgen Johansson; Bernt Eric Uhlin
Journal:  J Bacteriol       Date:  2006-08       Impact factor: 3.490

3.  Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection.

Authors:  T F Wei; T S Ramasubramanian; F Pu; J W Golden
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

4.  The structure of a CAP-DNA complex having two cAMP molecules bound to each monomer.

Authors:  J M Passner; T A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-01       Impact factor: 11.205

5.  The cyclic nucleotide monophosphate domain of Xanthomonas campestris global regulator Clp defines a new class of cyclic di-GMP effectors.

Authors:  Fei Tao; Ya-Wen He; Dong-Hui Wu; Sanjay Swarup; Lian-Hui Zhang
Journal:  J Bacteriol       Date:  2009-12-11       Impact factor: 3.490

6.  Mutagenesis of the cyclic AMP receptor protein of Escherichia coli: targeting positions 72 and 82 of the cyclic nucleotide binding pocket.

Authors:  A O Belduz; E J Lee; J G Harman
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

7.  Structural and energetic analysis of activation by a cyclic nucleotide binding domain.

Authors:  Stephen L Altieri; Gina M Clayton; William R Silverman; Adrian O Olivares; Enrique M De la Cruz; Lise R Thomas; João H Morais-Cabral
Journal:  J Mol Biol       Date:  2008-06-10       Impact factor: 5.469

8.  Carbon monoxide-induced activation of gene expression in Rhodospirillum rubrum requires the product of cooA, a member of the cyclic AMP receptor protein family of transcriptional regulators.

Authors:  D Shelver; R L Kerby; Y He; G P Roberts
Journal:  J Bacteriol       Date:  1995-04       Impact factor: 3.490

9.  Absence of direct cyclic nucleotide modulation of mEAG1 and hERG1 channels revealed with fluorescence and electrophysiological methods.

Authors:  Tinatin I Brelidze; Anne E Carlson; William N Zagotta
Journal:  J Biol Chem       Date:  2009-08-11       Impact factor: 5.157

10.  Roles of catabolite activator protein sites centered at -81.5 and -41.5 in the activation of the Klebsiella aerogenes histidine utilization operon hutUH.

Authors:  R Osuna; B K Janes; R A Bender
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490
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