| Literature DB >> 1333861 |
J E Corrie1, C Pizza, J Makwana, R W King.
Abstract
Reaction of cyclic AMP with 1,1'-carbonyldiimidazole produces an intermediate which reacts with primary amines to provide a stable 2'-O-carbamyl derivative. This chemistry has been used to tether cyclic AMP to a Sepharose gel. The resulting affinity support has been used to effect a simple, nondenaturing purification of cyclic AMP receptor protein from crude cell extracts.Entities:
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Year: 1992 PMID： 1333861 DOI： 10.1016/s1046-5928(05)80044-0
Source DB: PubMed Journal: Protein Expr Purif ISSN： 1046-5928 Impact factor: 1.650