| Literature DB >> 1333415 |
A Haddy1, S K Swanson, T L Born, F Rusnak.
Abstract
The interaction of the immunosuppressive complex cyclosporin A-cyclophilin (CsA-CyP) with the Ca2+/calmodulin-dependent protein phosphatase calcineurin is investigated using a recombinant form of the A subunit of calcineurin (rCNA). Only in the presence of purified calcineurin B (CNB) does rCNA show the response of native calcineurin, i.e. 50% inhibition of rCNA phosphatase activity at 6 nM human cyclophilin B and 0.6 microM human cyclophilin A using [32P]casein as substrate, yet stimulation of activity with p-nitrophenyl phosphate as substrate. This study demonstrates that the B subunit is necessary to confer sensitivity of calcineurin to CsA-CyP.Entities:
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Year: 1992 PMID： 1333415 DOI： 10.1016/0014-5793(92)81456-v
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124