Brain Na+/K(+)-ATPase regulation by serotonin and norepinephrine in normal and kindled rats.

In this work we confirmed the activation of rat brain Na+/K(+)-ATPase by norepinephrine (NE) and observed a variable response of the enzyme according to the brain region considered. In isolated neuronal or glial fractions from normal cerebral cortices, we studied the response of the enzyme to increasing concentrations of serotonin (5-HT) (10(-9)-10(-3) M). A dose-dependent response over basal values was present in glial fractions, beginning at 10(-6) M. No such response was obtained in the neuronal fractions. In amygdaloid kindled brains, the pattern of activation by NE was different than in controls: less pronounced (cortex, brainstem, and diencephalon), inhibition-activation (cerebellum), or no change (striatum). The activation of Na+/K(+)-ATPase by 5-HT observed in the control glial fraction was not present in the kindled glial fraction. In conclusion, 5-HT seems to activate Na+/K(+)-ATPase preferentially in glial cells, and the kindling process markedly modifies this regulation. The normal response to NE in brain homogenates is less altered by kindling than is the response to 5-HT in the same regions.