Literature DB >> 1333340

Effect of nitric oxide on mitogenesis and proliferation of cerebellar glial cells.

U C Garg1, L Devi, H Turndorf, L R Goldfrank, M Bansinath.   

Abstract

In the brain, nitric oxide (NO) has been identified as a messenger molecule and a mediator of excitatory amino acid-induced neurotoxicity. In this study, the effects of NO on serum-induced mitogenesis and cell proliferation of the cerebellar glial cells were assessed. NO-generating agent, S-nitroso-N-acetylpenicillamine (SNAP) increased intracellular cyclic guanosine monophosphate (cGMP) levels. Furthermore, 2 chemically dissimilar NO-generating agents, SNAP and sodium nitroprusside (SNP) inhibited serum-induced thymidine incorporation and cell proliferation. The antimitogenic effect of NO was mimicked by 8-bromo-cGMP and blocked by hemoglobin, a known inhibitor of NO. The effect of NO was not cytotoxic, since the cells were not stained with Trypan blue and did not show increased release of lactate dehydrogenase in the culture supernatants. However, NO-treated cells showed decreased conversion of tetrazolium to blue formazan suggesting that NO inhibited mitochondrial activity in the glial cells. These results demonstrate that NO inhibits serum-induced mitogenesis and cell proliferation of cultured rat cerebellar glial cells.

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Year:  1992        PMID: 1333340     DOI: 10.1016/0006-8993(92)91678-8

Source DB:  PubMed          Journal:  Brain Res        ISSN: 0006-8993            Impact factor:   3.252


  4 in total

1.  Nitric oxide reversibly inhibits the epidermal growth factor receptor tyrosine kinase.

Authors:  C Estrada; C Gómez; J Martín-Nieto; T De Frutos; A Jiménez; A Villalobo
Journal:  Biochem J       Date:  1997-09-01       Impact factor: 3.857

2.  Growth inhibition and radiosensitization of cultured glioma cells by nitric oxide generating agents.

Authors:  M Kurimoto; S Endo; Y Hirashima; H Hamada; T Ogiichi; A Takaku
Journal:  J Neurooncol       Date:  1999-03       Impact factor: 4.130

3.  Restoring soluble guanylyl cyclase expression and function blocks the aggressive course of glioma.

Authors:  Haifeng Zhu; Jessica Tao Li; Fang Zheng; Emil Martin; Alexander Y Kots; Joshua S Krumenacker; Byung-Kwon Choi; Ian E McCutcheon; Norman Weisbrodt; Oliver Bögler; Ferid Murad; Ka Bian
Journal:  Mol Pharmacol       Date:  2011-09-09       Impact factor: 4.436

4.  Chronic administration of a nitric oxide synthase inhibitor, N omega-nitro-L-arginine, and drug-induced increase in cerebellar cyclic GMP in vivo.

Authors:  M Bansinath; B Arbabha; H Turndorf; U C Garg
Journal:  Neurochem Res       Date:  1993-10       Impact factor: 3.996

  4 in total

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