Literature DB >> 1333295

Herbicide-resistant Indica rice plants from IRRI breeding line IR72 after PEG-mediated transformation of protoplasts.

S K Datta1, K Datta, N Soltanifar, G Donn, I Potrykus.   

Abstract

The commercially important Indica rice cultivar Oryza sativa cv. IR72 has been transformed using direct gene transfer to protoplasts. PEG-mediated transformation was done with two plasmid constructs containing either a CaMV 35S promoter/HPH chimaeric gene conferring resistance to hygromycin (Hg) or a CaMV 35S promoter/BAR chimaeric gene conferring resistance to a commercial herbicide (Basta) containing phosphinothricin (PPT). We have obtained so far 92 Hgr and 170 PPTr IR72 plants from protoplasts through selection. 31 Hgr and 70 PPTr plants are being grown in the greenhouse to maturity. Data from Southern analysis and enzyme assays proved that the transgene was stably integrated into the host genome and expressed. Transgenic plants showed complete resistance to high doses of the commercial formulations of PPT.

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Year:  1992        PMID： 1333295     DOI： 10.1007/bf00046447

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  30 in total

1.  The 3'-terminal region of the hygromycin-B-resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum.

Authors:  R Bilang; S Iida; A Peterhans; I Potrykus; J Paszkowski
Journal:  Gene       Date:  1991-04       Impact factor: 3.688

Review 2.  Transformation of plant cells via Agrobacterium.

Authors:  P J Hooykaas
Journal:  Plant Mol Biol       Date:  1989-09       Impact factor: 4.076

Review 3.  Direct DNA transfer to plant cells.

Authors:  M R Davey; E L Rech; B J Mulligan
Journal:  Plant Mol Biol       Date:  1989-09       Impact factor: 4.076

4.  Genetically transformed maize plants from protoplasts.

Authors:  C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal:  Science       Date:  1988-04-08       Impact factor: 47.728

5.  Rapid isolation of high molecular weight plant DNA.

Authors:  M G Murray; W F Thompson
Journal:  Nucleic Acids Res       Date:  1980-10-10       Impact factor: 16.971

6.  A polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants.

Authors:  A Hayashimoto; Z Li; N Murai
Journal:  Plant Physiol       Date:  1990-07       Impact factor: 8.340

7.  Transgenic plants as tools to study the molecular organization of plant genes.

Authors:  J St Schell
Journal:  Science       Date:  1987-09-04       Impact factor: 47.728

8.  Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants.

Authors:  D A Walters; C S Vetsch; D E Potts; R C Lundquist
Journal:  Plant Mol Biol       Date:  1992-01       Impact factor: 4.076

9.  Bialaphos selection of stable transformants from maize cell culture.

Authors:  T M Spencer; W J Gordon-Kamm; R J Daines; W G Start; P G Lemaux
Journal:  Theor Appl Genet       Date:  1990-05       Impact factor: 5.699

10.  Expression of a monocot LHCP promoter in transgenic rice.

Authors:  Y Tada; M Sakamoto; M Matsuoka; T Fujimura
Journal:  EMBO J       Date:  1991-07       Impact factor: 11.598
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  16 in total

1.  Gene transfer by electroporation into intact scutellum cells of wheat embryos.

Authors:  A Klöti; V A Iglesias; J Wünn; P K Burkhardt; S K Datta; I Potrykus
Journal:  Plant Cell Rep       Date:  1993-10       Impact factor: 4.570

2.  Transformation of haploid, microspore-derived cell suspension protoplasts of rice (Oryza sativa L.).

Authors:  H Chaïr; T Legavre; E Guiderdoni
Journal:  Plant Cell Rep       Date:  1996-06       Impact factor: 4.570

3.  Regeneration of fertile transgenic indica (group 1) rice plants following microprojectile transformation of embryogenic suspension culture cells.

Authors:  S Zhang; L Chen; R Qu; P Marmey; R Beachy; C Fauquet
Journal:  Plant Cell Rep       Date:  1996-03       Impact factor: 4.570

4.  Current developments in plant biotechnology for genetic improvement: the case of rice (Oryza sativa L.).

Authors:  F J Zapata-Arias; L B Torrizo; A Ando
Journal:  World J Microbiol Biotechnol       Date:  1995-07       Impact factor: 3.312

Review 5.  Transformation of rice mediated by Agrobacterium tumefaciens.

Authors:  Y Hiei; T Komari; T Kubo
Journal:  Plant Mol Biol       Date:  1997-09       Impact factor: 4.076

6.  Studies on growth dynamics of embryogenic cell suspension cultures of commercially important Indica rice cultivars ASD16 and Pusa basmati.

Authors:  Sundararajan Sathish; Rajendran Venkatesh; Nayeem Safia; Ramalingam Sathishkumar
Journal:  3 Biotech       Date:  2018-03-21       Impact factor: 2.406

Review 7.  Molecular improvement of cereals.

Authors:  I K Vasil
Journal:  Plant Mol Biol       Date:  1994-09       Impact factor: 4.076

8.  T-DNA integration into genomic DNA of rice following Agrobacterium inoculation of isolated shoot apices.

Authors:  S H Park; S R Pinson; R H Smith
Journal:  Plant Mol Biol       Date:  1996-12       Impact factor: 4.076

9.  Regeneration of transgenic plants from two indica rice (Oryza sativa L.) cultivars using shoot apex explants.

Authors:  S Arockiasamy; S Ignacimuthu
Journal:  Plant Cell Rep       Date:  2007-06-26       Impact factor: 4.570

10.  Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana.

Authors:  Jonni Koia; Richard Moyle; Caroline Hendry; Lionel Lim; José Ramón Botella
Journal:  Plant Mol Biol       Date:  2012-12-22       Impact factor: 4.076
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