Literature DB >> 1332307

Efficient electropulse transformation of intact Candida maltosa cells by different homologous vector plasmids.

A Kasüske1, H Wedler, S Schulze, D Becher.   

Abstract

Conditions for efficient and quick transformation by electroporation were developed in Candida maltosa. To investigate the efficiency of transformation with integrative as well as with autonomously replicating plasmids, a series of vectors was constructed for homologous transformation of this species. Transformants were obtained with different plasmids as covalently closed circular molecules and as linearized DNA. The influence of recipient strain and plasmid type as well as of cell number and parameters of the supplied electrical pulse on the transformation efficiency have been investigated. A maximum of 7000 transformants per 100 ng of plasmid DNA was reached. The efficiency of transformation was compared with that of the LiCl method.

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Year:  1992        PMID： 1332307     DOI： 10.1002/yea.320080902

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  7 in total

1.  Transformation of Kluyveromyces lactis by Electroporation.

Authors:  M Sánchez; F J Iglesias; C Santamaría; A Domínguez
Journal:  Appl Environ Microbiol       Date:  1993-07       Impact factor: 4.792

2.  Identification of yeast associated with the planthopper, Perkinsiella saccharicida: potential applications for Fiji leaf gall control.

Authors:  Grant L Hughes; Peter G Allsopp; Richard I Webb; Ryuichi Yamada; Inaki Iturbe-Ormaetxe; Stevens M Brumbley; Scott L O'Neill
Journal:  Curr Microbiol       Date:  2011-08-18       Impact factor: 2.188

Review 3.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

4.  Highly-efficient electrotransformation of the yeast Hansenula polymorpha.

Authors:  K N Faber; P Haima; W Harder; M Veenhuis; G AB
Journal:  Curr Genet       Date:  1994-04       Impact factor: 3.886

5.  A transformation system for the yeast Candida utilis: use of a modified endogenous ribosomal protein gene as a drug-resistant marker and ribosomal DNA as an integration target for vector DNA.

Authors:  K Kondo; T Saito; S Kajiwara; M Takagi; N Misawa
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

6.  Molecular analysis of a leu2-mutant of Candida maltosa demonstrates the presence of multiple alleles.

Authors:  D Becher; S Schulze; A Kasüske; H Schulze; I A Samsonova; S G Oliver
Journal:  Curr Genet       Date:  1994-09       Impact factor: 3.886

7.  Chromosome polymorphisms close to the cm-ADE1 locus of candida maltosa.

Authors:  D Becher; S Schulze; A Kasüske; R Stoll; H Wedler; S G Oliver
Journal:  Mol Gen Genet       Date:  1995-06-10
  7 in total

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