| Literature DB >> 1331157 |
M Y Liu1, C Y Pai, S M Shieh, T Y Hsu, J Y Chen, C S Yang.
Abstract
A clone of the Epstein-Barr virus (EBV) thymidine kinase (TK) gene was derived from a cDNA library of P3HR1 cells. The gene product was expressed as a fusion protein in a procaryotic system by using T7 RNA polymerase. The recombinant TK showed a molecular mass of 67 kDa and was biologically active. Antiserum raised in mice immunized with partially purified TK recognized an antigen present in EBV-superinfected Raji cells using an indirect immunofluorescence assay.Entities:
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Year: 1992 PMID： 1331157 DOI： 10.1016/0166-0934(92)90012-3
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014