Literature DB >> 1330133

Heterologous expression of recombinant human glutathione transferase A1-1 from a hepatoma cell line.

G Stenberg1, R Björnestedt, B Mannervik.   

Abstract

A cDNA clone, lambda GTHA1, encoding human glutathione transferase A1-1 has been isolated from a hepatoma HepG2 cDNA library. At the nucleotide level, the new clone showed minor differences from cDNA deriving from normal liver, but the deduced amino acid sequence was identical to the structure previously described. The protein was expressed from a plasmid, pKHA1, and isolated by a single-step affinity purification on an S-hexylglutathione Sepharose matrix. The yield of the recombinant protein was 165 mg from a 3-liter culture of bacteria.

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Year:  1992        PMID： 1330133     DOI： 10.1016/1046-5928(92)90060-a

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  14 in total

1.  Impact of domain interchange on conformational stability and equilibrium folding of chimeric class micro glutathione transferases.

Authors:  Jiann-Kae Luo; Judith A T Hornby; Louise A Wallace; Jihong Chen; Richard N Armstrong; Heini W Dirr
Journal:  Protein Sci       Date:  2002-09       Impact factor: 6.725

2.  The role of a topologically conserved isoleucine in glutathione transferase structure, stability and function.

Authors:  Ikechukwu Achilonu; Samantha Gildenhuys; Loren Fisher; Jonathan Burke; Sylvia Fanucchi; B Trevor Sewell; Manuel Fernandes; Heini W Dirr
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-06-23

3.  Human glutathione transferase A4-4: an alpha class enzyme with high catalytic efficiency in the conjugation of 4-hydroxynonenal and other genotoxic products of lipid peroxidation.

Authors:  I Hubatsch; M Ridderström; B Mannervik
Journal:  Biochem J       Date:  1998-02-15       Impact factor: 3.857

4.  Phospholipid hydroperoxide glutathione peroxidase activity of human glutathione transferases.

Authors:  R Hurst; Y Bao; P Jemth; B Mannervik; G Williamson
Journal:  Biochem J       Date:  1998-05-15       Impact factor: 3.857

5.  Studies on the differential inhibition of glutathione conjugate formation of (+)-anti-benzo[a]pyrene 7,8-dihydrodiol 9,10-epoxide and 1-chloro-2,4-dinitrobenzene in V79 Chinese hamster cells.

Authors:  K Sundberg; B Jernström; S Swedmark
Journal:  Biochem J       Date:  2000-08-01       Impact factor: 3.857

6.  Redesign of substrate-selectivity determining modules of glutathione transferase A1-1 installs high catalytic efficiency with toxic alkenal products of lipid peroxidation.

Authors:  L O Nilsson; A Gustafsson; B Mannervik
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-15       Impact factor: 11.205

7.  A topologically conserved aliphatic residue in alpha-helix 6 stabilizes the hydrophobic core in domain II of glutathione transferases and is a structural determinant for the unfolding pathway.

Authors:  L A Wallace; G L Blatch; H W Dirr
Journal:  Biochem J       Date:  1998-12-01       Impact factor: 3.857

8.  The role of an evolutionarily conserved cis-proline in the thioredoxin-like domain of human class Alpha glutathione transferase A1-1.

Authors:  Chris Nathaniel; Louise A Wallace; Jonathan Burke; Heini W Dirr
Journal:  Biochem J       Date:  2003-05-15       Impact factor: 3.857

9.  Isolation and characterization of the Methylophilus sp. strain DM11 gene encoding dichloromethane dehalogenase/glutathione S-transferase.

Authors:  R Bader; T Leisinger
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

10.  Characterization of bromosulphophthalein binding to human glutathione S-transferase A1-1: thermodynamics and inhibition kinetics.

Authors:  Doris Kolobe; Yasien Sayed; Heini W Dirr
Journal:  Biochem J       Date:  2004-09-01       Impact factor: 3.857
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