| Literature DB >> 1330043 |
A Y Chikishev1, G W Lucassen, N I Koroteev, C Otto, J Greve.
Abstract
Polarization sensitive coherent anti-Stokes Raman scattering (PCARS) spectroscopy is a fruitful technique to study Raman vibrations of diluted molecules under off-electron resonant conditions. We apply PCARS as a direct spectroscopic method to investigate the broad amide I band of proteins in heavy water. In spontaneous Raman spectroscopy, this band is not well resolved. We fit a number of spectra taken of each protein under different polarization conditions, with a single set of parameters. It then appears that some substructure is observed in the amide I band. From this substructure, we determine the percentage of alpha-helix, beta-sheet, and random coil for the proteins lysozyme, albumin, ribonuclease A, and alpha-chymotrypsin.Entities:
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Year: 1992 PMID： 1330043 PMCID： PMC1262235 DOI： 10.1016/S0006-3495(92)81691-0
Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033