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Literature DB >> 1329095

A 5' to 3' exonuclease functionally interacts with calf DNA polymerase epsilon.

G Siegal¹, J J Turchi, T W Myers, R A Bambara.

Abstract

Analysis of fractions containing purified DNA polymerase epsilon from calf thymus has revealed the presence of a 5' to 3' exonuclease activity that is specific for a single strand of duplex DNA. This activity is capable of degrading a 3'-labeled oligonucleotide hybridized to M13mp18 DNA. When a second oligonucleotide primer is annealed 3 bases upstream, degradation of the downstream primer is strictly dependent on DNA synthesis from the upstream primer. Replacement of the downstream primer by an oligoribonucleotide of identical sequence results in a similar pattern of exonucleolytic activity. The activity has been highly purified and found to cosediment in glycerol gradients with a peptide of 56 kDa as judged by SDS/PAGE analysis. Effects of calf DNA polymerase alpha and delta on exonuclease activity are also observed but with differences in the pattern of products.

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Year: 1992 PMID： 1329095 PMCID： PMC50134 DOI： 10.1073/pnas.89.20.9377

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

25 in total

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Authors: R A Bambara; C B Jessee
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2. Discontinuous DNA synthesis by purified mammalian proteins.

Authors: M Goulian; S H Richards; C J Heard; B M Bigsby
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3. Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin.

Authors: T Tsurimoto; T Melendy; B Stillman
Journal: Nature Date: 1990-08-09 Impact factor: 49.962

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Authors: S H Lee; Z Q Pan; A D Kwong; P M Burgers; J Hurwitz
Journal: J Biol Chem Date: 1991-11-25 Impact factor: 5.157

5. Influence of poly(ADP-ribose) polymerase on the enzymatic synthesis of SV40 DNA.

Authors: T Eki; J Hurwitz
Journal: J Biol Chem Date: 1991-02-15 Impact factor: 5.157

6. An active fragment of DNA polymerase produced by proteolytic cleavage.

Authors: D Brutlag; M R Atkinson; P Setlow; A Kornberg
Journal: Biochem Biophys Res Commun Date: 1969-12-04 Impact factor: 3.575

7. A third essential DNA polymerase in S. cerevisiae.

Authors: A Morrison; H Araki; A B Clark; R K Hamatake; A Sugino
Journal: Cell Date: 1990-09-21 Impact factor: 41.582

8. Substrate specificity of the exonuclease associated with calf DNA polymerase.

Authors: R D Sabatino; T W Myers; R A Bambara
Journal: Cancer Res Date: 1990-09-01 Impact factor: 12.701

9. Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

Authors: P M Holland; R D Abramson; R Watson; D H Gelfand
Journal: Proc Natl Acad Sci U S A Date: 1991-08-15 Impact factor: 11.205

10. Structural relationships between two forms of DNA polymerase epsilon from calf thymus.

Authors: G Siegal; J J Turchi; C B Jessee; L M Mallaber; R A Bambara; T W Myers
Journal: J Biol Chem Date: 1992-02-25 Impact factor: 5.157

20 in total

1. Comparison of the 5' nuclease activities of taq DNA polymerase and its isolated nuclease domain.

Authors: V Lyamichev; M A Brow; V E Varvel; J E Dahlberg
Journal: Proc Natl Acad Sci U S A Date: 1999-05-25 Impact factor: 11.205

2. Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells.

Authors: Bochao Liu; Jiazhi Hu; Jingna Wang; Daochun Kong
Journal: J Biol Chem Date: 2017-02-03 Impact factor: 5.157

3. Enzymatic completion of mammalian lagging-strand DNA replication.

Authors: J J Turchi; L Huang; R S Murante; Y Kim; R A Bambara
Journal: Proc Natl Acad Sci U S A Date: 1994-10-11 Impact factor: 11.205

4. Heterogeneity of primer extension products in asymmetric PCR is due both to cleavage by a structure-specific exo/endonuclease activity of DNA polymerases and to premature stops.

Authors: G Tombline; D Bellizzi; V Sgaramella
Journal: Proc Natl Acad Sci U S A Date: 1996-04-02 Impact factor: 11.205

9. Lagging strand DNA synthesis by calf thymus DNA polymerases alpha, beta, delta and epsilon in the presence of auxiliary proteins.

Authors: V N Podust; U Hübscher
Journal: Nucleic Acids Res Date: 1993-02-25 Impact factor: 16.971

10. Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair gene.

Authors: M S Reagan; C Pittenger; W Siede; E C Friedberg
Journal: J Bacteriol Date: 1995-01 Impact factor: 3.490