Literature DB >> 1329033

Disulfide bonds are required for Serratia marcescens nuclease activity.

T K Ball1, Y Suh, M J Benedik.   

Abstract

The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

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Year:  1992        PMID： 1329033      PMCID： PMC334271          DOI： 10.1093/nar/20.19.4971

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

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Authors:  A J Doig; D H Williams
Journal:  J Mol Biol       Date:  1991-01-20       Impact factor: 5.469

2.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
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Review 3.  The ins and outs of colicins. Part II. Lethal action, immunity and ecological implications.

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Journal:  Microbiol Sci       Date:  1984-11

4.  The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli.

Authors:  T K Ball; P N Saurugger; M J Benedik
Journal:  Gene       Date:  1987       Impact factor: 3.688

5.  Production of active Serratia marcescens metalloprotease from Escherichia coli by alpha-hemolysin HlyB and HlyD.

Authors:  Y Suh; M J Benedik
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

6.  Specific excretion of Serratia marcescens protease through the outer membrane of Escherichia coli.

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Authors:  L Q Zhu; T Gangopadhyay; K P Padmanabha; M P Deutscher
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8.  Purification and characterization of a Serratia marcescens nuclease produced by Escherichia coli.

Authors:  K Biedermann; P K Jepsen; E Riise; I Svendsen
Journal:  Carlsberg Res Commun       Date:  1989

9.  Identification of a protein required for disulfide bond formation in vivo.

Authors:  J C Bardwell; K McGovern; J Beckwith
Journal:  Cell       Date:  1991-11-01       Impact factor: 41.582

10.  Regulatory mutants and transcriptional control of the Serratia marcescens extracellular nuclease gene.

Authors:  Y C Chen; G L Shipley; T K Ball; M J Benedik
Journal:  Mol Microbiol       Date:  1992-03       Impact factor: 3.501
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  14 in total

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2.  The extracellular nuclease of Serratia marcescens: studies on the activity in vitro and effect on transforming DNA in a groundwater aquifer microcosm.

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Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552

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4.  Secretion of nuclease across the outer membrane of Serratia marcescens and its energy requirements.

Authors:  Y Suh; M J Benedik
Journal:  J Bacteriol       Date:  1997-02       Impact factor: 3.490

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6.  Differential secretion of isoforms of Serratia marcescens extracellular nuclease.

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Journal:  Appl Environ Microbiol       Date:  1995-11       Impact factor: 4.792

7.  The impact of different methods of DNA extraction on microbial community measures of BALF samples based on metagenomic data.

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8.  Two-step secretion of the Serratia marcescens extracellular nuclease.

Authors:  Y Suh; S Jin; T K Ball; M J Benedik
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

9.  Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis.

Authors:  P Friedhoff; O Gimadutdinow; A Pingoud
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10.  Crystal structure of the EndoG/EndoGI complex: mechanism of EndoG inhibition.

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Journal:  Nucleic Acids Res       Date:  2009-11       Impact factor: 16.971
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