| Literature DB >> 1328123 |
K Yoshizawa1, K Kiyosawa, S Usuda, K Yabu, Y Nakatsuji, S Yamada, K Furuta, E Tanaka, T Sodeyama, S Furuta.
Abstract
A new human hepatocellular carcinoma cell (HCC) line, designated SUHC-1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV-RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase-contrast and electron microscopy. The SUHC-1 cells produced albumin and alpha 2-macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p-, 1q-, i(1q), i(17q) and 20q+) with a modal number of 91. HCV-RNA was not detected in the supernatant of SUHC-1 cells by nested polymerase chain reaction assay or in the SUHC-1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC-1 cell line. The SUHC-1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV.Entities:
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Year: 1992 PMID： 1328123 PMCID： PMC5918961 DOI： 10.1111/j.1349-7006.1992.tb01993.x
Source DB: PubMed Journal: Jpn J Cancer Res ISSN： 0910-5050