Literature DB >> 1327290

A rapid method for starting a culture for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines.

Y Fukushima1, H Ohashi, K Wakui, T Nishida, T Oh-ishi.   

Abstract

We developed a simple and efficient procedure for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines. B-lymphocytes were obtained by centrifugation after hemolysis of red cells with a hemolysis buffer, instead of Ficoll-Parque gradient. We can start a primary culture within 15 min by using this method.

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Year:  1992        PMID： 1327290     DOI： 10.1007/BF01899737

Source DB:  PubMed          Journal:  Jpn J Hum Genet        ISSN： 0916-8478


  8 in total

1.  Lentiviral calnexin-modified dendritic cells promote expansion of high-avidity effector T cells with central memory phenotype.

Authors:  Bei Wang; Shuhong Han; Lily Lien; Lung-Ji Chang
Journal:  Immunology       Date:  2009-09       Impact factor: 7.397

2.  A type II collagen mutation also results in oto-spondylo-megaepiphyseal dysplasia.

Authors:  Yoshinari Miyamoto; Eiji Nakashima; Hisatada Hiraoka; Hirofumi Ohashi; Shiro Ikegawa
Journal:  Hum Genet       Date:  2005-11-15       Impact factor: 4.132

3.  Mutations of the fibroblast growth factor receptor-3 gene in one familial and six sporadic cases of achondroplasia in Japanese patients.

Authors:  S Ikegawa; Y Fukushima; M Isomura; F Takada; Y Nakamura
Journal:  Hum Genet       Date:  1995-09       Impact factor: 4.132

4.  Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications.

Authors:  Shuhong Han; Yuju Huang; Yin Liang; Yuchin Ho; Yichen Wang; Lung-Ji Chang
Journal:  J Hematol Oncol       Date:  2009-08-06       Impact factor: 17.388

5.  A stable acentric marker chromosome: possible existence of an intercalary ancient centromere at distal 8p.

Authors:  H Ohashi; K Wakui; K Ogawa; T Okano; N Niikawa; Y Fukushima
Journal:  Am J Hum Genet       Date:  1994-12       Impact factor: 11.025

6.  Detection of a cryptic paracentric inversion within band 11p13 in familial aniridia by fluorescence in situ hybridization.

Authors:  Y Fukushima; J Hoovers; M Mannens; K Wakui; H Ohashi; T Ohno; Y Ueoka; N Niikawa
Journal:  Hum Genet       Date:  1993-04       Impact factor: 4.132

7.  Efficient and reliable establishment of lymphoblastoid cell lines by Epstein-Barr virus transformation from a limited amount of peripheral blood.

Authors:  Natsue Omi; Yuichi Tokuda; Yoko Ikeda; Morio Ueno; Kazuhiko Mori; Chie Sotozono; Shigeru Kinoshita; Masakazu Nakano; Kei Tashiro
Journal:  Sci Rep       Date:  2017-03-08       Impact factor: 4.379

8.  Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT.

Authors:  Hiroaki Murakami; Norito Tamura; Yumi Enomoto; Kentaro Shimasaki; Kenji Kurosawa; Kentaro Hanada
Journal:  PLoS One       Date:  2020-12-21       Impact factor: 3.240
  8 in total

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