Literature DB >> 13263327

Buffered osmium tetroxide (OsO4) fixation for cytological and Feulgen microspectrophotometric studies of human rectal polyps.

C LEUCHTENBERGER, P F DOOLIN, A H KUTSAKIS.   

Abstract

Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO(4) according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also can be carried out successfully and such OsO(4)-fixed Feulgen-stained sections are especially suitable for microspectrophotometric determinations of DNA in individual nuclei.

Entities:  

Keywords:  OSMIUM; POLYPI; RECTUM/neoplasms

Mesh：

Substances：

Year:  1955        PMID： 13263327      PMCID： PMC2229658          DOI： 10.1083/jcb.1.5.385

Source DB:  PubMed          Journal:  J Biophys Biochem Cytol        ISSN： 0095-9901


  4 in total

1.  Critical evaluation of Feulgen microspectrophotometry for estimating amount of DNA in cell nuclei.

Authors:  C LEUCHTENBERGER
Journal:  Science       Date:  1954-12-17       Impact factor: 47.728

2.  Cytoplasmic "inclusion bodies" containing desoxyribose nucleic acid (DNA) in cells of human rectal polyps.

Authors:  C LEUCHTENBERGER
Journal:  Lab Invest       Date:  1954 Mar-Apr       Impact factor: 5.662

3.  A cytochemical study of pycnotic nuclear degeneration.

Authors:  C LEUCHTENBERGER
Journal:  Chromosoma       Date:  1950       Impact factor: 4.316

4.  A study of fixation for electron microscopy.

Authors:  G E PALADE
Journal:  J Exp Med       Date:  1952-03       Impact factor: 14.307
  4 in total
  4 in total

1.  [On the fine structure of chromosomes. I. On the cytology of Escherichia coli. B. Cell growth, importance of electronoptic findings].

Authors:  F W SCHLOTE
Journal:  Arch Mikrobiol       Date:  1961

2.  Studies on nuclei using correlated cytochemical, light, and electron microscope techniques.

Authors:  M J MOSES
Journal:  J Biophys Biochem Cytol       Date:  1956-07-25

3.  Staining methods applicable to sections of osmium-fixed tissue for light microscopy.

Authors:  B L MUNGER
Journal:  J Biophys Biochem Cytol       Date:  1961-11

4.  A correlated histochemical and electron microscopic study of the intranuclear crystalline aggregates of adenovirus (RI-APC virus) in HeLa cells.

Authors:  D P BLOCH; C MORGAN; G C GODMAN; C HOWE; H M ROSE
Journal:  J Biophys Biochem Cytol       Date:  1957-01-25
  4 in total

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