Literature DB >> 1325780

Expression in Escherichia coli and characterization of a recombinant 7Fe ferredoxin of Rhodobacter capsulatus.

Y Jouanneau1, C Duport, C Meyer, J Gaillard.   

Abstract

The 7Fe ferredoxin of Rhodobacter capsulatus (FdII) could be expressed in Escherichia coli by cloning the fdxA gene coding for FdII downstream from the lac promoter. The expressed recombinant ferredoxin appeared as a brown protein which was specifically recognized in E. coli cell-free extracts by anti-FdII serum. The purified recombinant ferredoxin was indistinguishable from R. capsulatus FdII on the basis of its molecular, redox and spectroscopic properties. These results indicate that the [3Fe-4S] and [4Fe-4S] clusters were correctly inserted into the recombinant ferredoxin.

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Year:  1992        PMID： 1325780      PMCID： PMC1133050          DOI： 10.1042/bj2860269

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  Primary structure of a 7Fe ferredoxin from Streptomyces griseus.

Authors:  M K Trower; J E Marshall; M S Doleman; M H Emptage; F S Sariaslani
Journal:  Biochim Biophys Acta       Date:  1990-03-01

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Characterization of the recombinant Clostridium pasteurianum ferredoxin and comparison of its properties with those of the native protein.

Authors:  J R Baur; M C Graves; B A Feinberg; S W Ragsdale
Journal:  Biofactors       Date:  1990-07       Impact factor: 6.113

4.  Structure of ferredoxin I from Azotobacter vinelandii.

Authors:  G H Stout; S Turley; L C Sieker; L H Jensen
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

5.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Maturation of the head of bacteriophage T4. I. DNA packaging events.

Authors:  U K Laemmli; M Favre
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469

7.  Refinement of the 7 Fe ferredoxin from Azotobacter vinelandii at 1.9 A resolution.

Authors:  C D Stout
Journal:  J Mol Biol       Date:  1989-02-05       Impact factor: 5.469

8.  Transcriptional analysis and promoter mapping of the fdxA gene which encodes the 7Fe ferredoxin (FdII) of Rhodobacter capsulatus.

Authors:  C Duport; Y Jouanneau; P M Vignais
Journal:  Mol Gen Genet       Date:  1992-01

9.  Expression of human ferredoxin and assembly of the [2Fe-2S] center in Escherichia coli.

Authors:  V M Coghlan; L E Vickery
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

10.  Large-scale purification of high activity Azotobacter vinelandII nitrogenase.

Authors:  B K Burgess; D B Jacobs; E I Stiefel
Journal:  Biochim Biophys Acta       Date:  1980-07-10
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  3 in total

1.  Expression in Escherichia coli and characterization of a reconstituted recombinant 7Fe ferredoxin from Desulfovibrio africanus.

Authors:  J L Busch; J L Breton; B M Bartlett; R James; E C Hatchikian; A J Thomson
Journal:  Biochem J       Date:  1996-02-15       Impact factor: 3.857

2.  Recombinant expression of the fdxD gene of Rhodobacter capsulatus and characterization of its product, a [2Fe-2S] ferredoxin.

Authors:  J Armengaud; C Meyer; Y Jouanneau
Journal:  Biochem J       Date:  1994-06-01       Impact factor: 3.857

3.  Sequence, assembly and evolution of a primordial ferredoxin from Thermotoga maritima.

Authors:  B Darimont; R Sterner
Journal:  EMBO J       Date:  1994-04-15       Impact factor: 11.598
  3 in total

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