Literature DB >> 1324847

Ca2+/calmodulin-activated protein phosphatase (PP2B) of Saccharomyces cerevisiae. PP2B activity is not essential for growth.

T Nakamura1, H Tsutsumi, H Mukai, T Kuno, T Miyakawa.   

Abstract

Protein phosphatase (PP2B) whose activity is stimulated 12-20-fold by Ca2+/calmodulin (CaM) was partially purified by CaM-Sepharose and heparin-agarose chromatographies from cell extract of the yeast Saccharomyces cerevisiae. PP2B activity was not detectable in a mutant in which two genes (CMP1 and CMP2) encoding homologs of mammalian PP2B catalytic subunit were disrupted. We have previously shown that the double gene disruption has no significant effect on the growth of yeast [1991, Mol. Gen. Genet. 227, 52-59]. The results indicated that CMP1 and CMP2 are the only genes that encode the PP2B catalytic polypeptide in S. cerevisiae, and PP2B activity is not essential for the growth of the yeast under normal conditions.

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Year:  1992        PMID： 1324847     DOI： 10.1016/0014-5793(92)80749-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  7 in total

Review 1.  Serine/threonine protein phosphatases.

Authors:  S Wera; B A Hemmings
Journal:  Biochem J       Date:  1995-10-01       Impact factor: 3.857

2.  Genetic evidence for the functional redundancy of the calcineurin- and Mpk1-mediated pathways in the regulation of cellular events important for growth in Saccharomyces cerevisiae.

Authors:  T Nakamura; T Ohmoto; D Hirata; E Tsuchiya; T Miyakawa
Journal:  Mol Gen Genet       Date:  1996-05-23

3.  Calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, is essential in yeast mutants with cell integrity defects and in mutants that lack a functional vacuolar H(+)-ATPase.

Authors:  P Garrett-Engele; B Moilanen; M S Cyert
Journal:  Mol Cell Biol       Date:  1995-08       Impact factor: 4.272

4.  Calcineurin is essential in cyclosporin A- and FK506-sensitive yeast strains.

Authors:  T Breuder; C S Hemenway; N R Movva; M E Cardenas; J Heitman
Journal:  Proc Natl Acad Sci U S A       Date:  1994-06-07       Impact factor: 11.205

5.  Differential expression and function of two homologous subunits of yeast 1,3-beta-D-glucan synthase.

Authors:  P Mazur; N Morin; W Baginsky; M el-Sherbeini; J A Clemas; J B Nielsen; F Foor
Journal:  Mol Cell Biol       Date:  1995-10       Impact factor: 4.272

6.  Targets of immunophilin-immunosuppressant complexes are distinct highly conserved regions of calcineurin A.

Authors:  M E Cardenas; R S Muir; T Breuder; J Heitman
Journal:  EMBO J       Date:  1995-06-15       Impact factor: 11.598

7.  Protein phosphatase type 2B (calcineurin)-mediated, FK506-sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions.

Authors:  T Nakamura; Y Liu; D Hirata; H Namba; S Harada; T Hirokawa; T Miyakawa
Journal:  EMBO J       Date:  1993-11       Impact factor: 11.598
  7 in total

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