Ion transport by the isolated mantle epithelium of the freshwater clam, Unio complanatus.

Transepithelial ion transport was studied in three types of shell-facing mantle epithelia of the freshwater clam, Unio complanatus. These were 1) the epithelium isolated from near the margin of the shell; 2) the epithelium from the central region of a "normal" shell; and 3) the epithelium from the central region that was regenerating a damaged shell. Marginal and "shell-regenerating" epithelia produced a small (less than 5 mV) transepithelial voltage that was independent of PCO2 (0-4%). In the presence of 4% PCO2, the "normal" central epithelium produced a significantly larger transepithelial voltage (-8.5 +/- 0.99 mV), that, in addition, could be stimulated (-30.5 +/- 2.18 mV) by permeable analogues of adenosine 3',5'-cyclic monophosphate (cAMP) [e.g., 8-(4-chlorophenylthio)-adenosine 3',5'-cyclic monophosphate]. Voltage clamping the transepithelial voltage to zero resulted in a short-circuit current (Isc) that was consistent with positive charge moving from the hemolymph toward the shell. The measurements of transepithelial fluxes of 45Ca, 22Na, 42K, and 36Cl revealed that none of these ions, alone or in combination, could account for the stimulated Isc in "normal" mantle. In addition, similar measurements in marginal and "shell-regenerating" epithelia did not detect any significant net transepithelial flux of any of these ions. Acid extrusion at the shell-facing membrane and bicarbonate extrusion at the hemolymph-facing membrane were identified in "normal" central epithelium, but could not be found in marginal or "shell-regenerating" epithelia. These fluxes are equivalent to the cAMP-stimulated Isc in "normal" central epithelium.(ABSTRACT TRUNCATED AT 250 WORDS)