Literature DB >> 1320613

Fis plays a role in Tn5 and IS50 transposition.

M D Weinreich1, W S Reznikoff.   

Abstract

The Fis (factor for inversion stimulation) protein of Escherichia coli was found to influence the frequency of transposon Tn5 and insertion sequence IS50 transposition. Fis stimulated both Tn5 and IS50 transposition events and also inhibited IS50 transposition in Dam-bacteria. This influence was not due to regulation by Fis of the expression of the Tn5 transposition proteins. We localized, by DNase I footprinting, one Fis site overlapping the inside end of IS50 and give evidence to strongly suggest that when Fis binds to this site, IS50 transposition is inhibited. The Fis site at the inside end overlaps three Dam GATC sites, and Fis bound efficiently only to the unmethylated substrate. Using a mobility shift assay, we also identified another potential Fis site within IS50. Given the growth phase-dependent expression of Fis and its differential effect on Tn5 versus IS50 transposition in Dam-bacteria, we propose that the high levels of Fis present during exponential growth stimulate transposition events and might bias those events toward Tn5 and away from IS50 transposition.

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Year:  1992        PMID： 1320613      PMCID： PMC206248          DOI： 10.1128/jb.174.14.4530-4537.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  36 in total

1.  Translation initiation of IS50R read-through transcripts.

Authors:  V P Schulz; W S Reznikoff
Journal:  J Mol Biol       Date:  1991-09-05       Impact factor: 5.469

2.  A sequence at the inside end of IS50 down regulates transposition.

Authors:  K W Dodson; D E Berg
Journal:  Plasmid       Date:  1991-03       Impact factor: 3.466

3.  The N-terminal part of the E.coli DNA binding protein FIS is essential for stimulating site-specific DNA inversion but is not required for specific DNA binding.

Authors:  C Koch; O Ninnemann; H Fuss; R Kahmann
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

4.  Multiple effects of Fis on integration and the control of lysogeny in phage lambda.

Authors:  C A Ball; R C Johnson
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

5.  Efficient excision of phage lambda from the Escherichia coli chromosome requires the Fis protein.

Authors:  C A Ball; R C Johnson
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

6.  Three-dimensional structure of the E. coli DNA-binding protein FIS.

Authors:  D Kostrewa; J Granzin; C Koch; H W Choe; S Raghunathan; W Wolf; J Labahn; R Kahmann; W Saenger
Journal:  Nature       Date:  1991-01-10       Impact factor: 49.962

7.  Copy number control of Tn5 transposition.

Authors:  R C Johnson; W S Reznikoff
Journal:  Genetics       Date:  1984-05       Impact factor: 4.562

8.  Transposition of R factor genes to bacteriophage lambda.

Authors:  D E Berg; J Davies; B Allet; J D Rochaix
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

9.  The role of FIS in trans activation of stable RNA operons of E. coli.

Authors:  L Nilsson; A Vanet; E Vijgenboom; L Bosch
Journal:  EMBO J       Date:  1990-03       Impact factor: 11.598

10.  Identification of two functional regions in Fis: the N-terminus is required to promote Hin-mediated DNA inversion but not lambda excision.

Authors:  R Osuna; S E Finkel; R C Johnson
Journal:  EMBO J       Date:  1991-06       Impact factor: 11.598
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  25 in total

1.  Activation of Escherichia coli leuV transcription by FIS.

Authors:  W Ross; J Salomon; W M Holmes; R L Gourse
Journal:  J Bacteriol       Date:  1999-06       Impact factor: 3.490

2.  Involvement of H-NS in transpositional recombination mediated by IS1.

Authors:  Y Shiga; Y Sekine; Y Kano; E Ohtsubo
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

Review 3.  Roles of DNA adenine methylation in regulating bacterial gene expression and virulence.

Authors:  D A Low; N J Weyand; M J Mahan
Journal:  Infect Immun       Date:  2001-12       Impact factor: 3.441

4.  Bacterial luciferase activity and the intracellular redox pool in Escherichia coli.

Authors:  K Koga; T Harada; H Shimizu; K Tanaka
Journal:  Mol Genet Genomics       Date:  2005-10-11       Impact factor: 3.291

5.  The role of the local environment of engineered Tyr to Trp substitutions for probing the denaturation mechanism of FIS.

Authors:  Virginia A Muñiz; Saipraveen Srinivasan; Sarah A Boswell; Derrick W Meinhold; Tawanna Childs; Robert Osuna; Wilfredo Colón
Journal:  Protein Sci       Date:  2011-02       Impact factor: 6.725

6.  Functional characterization of the Escherichia coli Fis-DNA binding sequence.

Authors:  Yongping Shao; Leah S Feldman-Cohen; Robert Osuna
Journal:  J Mol Biol       Date:  2007-12-07       Impact factor: 5.469

7.  Fis regulates transcriptional induction of RpoS in Salmonella enterica.

Authors:  Matthew Hirsch; Thomas Elliott
Journal:  J Bacteriol       Date:  2005-03       Impact factor: 3.490

8.  Examination of the Tn5 transposase overproduction phenotype in Escherichia coli and localization of a suppressor of transposase overproduction killing that is an allele of rpoH.

Authors:  H Yigit; W S Reznikoff
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

9.  Escherichia coli DNA topoisomerase I and suppression of killing by Tn5 transposase overproduction: topoisomerase I modulates Tn5 transposition.

Authors:  H Yigit; W S Reznikoff
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

Review 10.  Insertion sequences.

Authors:  J Mahillon; M Chandler
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056
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