Literature DB >> 1318623

Expression of the gene coding for the major outer capsid protein of SA-11 rotavirus in a baculovirus system.

T P McGonigal1, M C Turon, K Komar-Hartnett, S E Kister, R E Smith.   

Abstract

The gene coding for the major outer capsid protein (VP7) of simian rotavirus SA-11 has been expressed in a baculovirus-insect cell system. The resulting protein is 35 kDa and is primarily associated with the endoplasmic reticulum. Neutralizing SA-11 polyclonal antiserum and VP7 monospecific antiserum reacted specifically with the expressed gene product. Antiserum derived against the recombinant VP7 protein neutralized SA-11 rotavirus infectivity in a fluorescent focus assay.

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Year:  1992        PMID: 1318623     DOI: 10.1016/0168-1702(92)90073-i

Source DB:  PubMed          Journal:  Virus Res        ISSN: 0168-1702            Impact factor:   3.303


  6 in total

1.  Expression of two bovine rotavirus non-structural proteins (NSP2, NSP3) in the baculovirus system and production of monoclonal antibodies directed against the expressed proteins.

Authors:  C Aponte; N M Mattion; M K Estes; A Charpilienne; J Cohen
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

2.  Synthesis of a ricin toxin B subunit-rotavirus VP7 fusion protein in potato.

Authors:  Nak-Won Choi; Mary K Estes; William H R Langridge
Journal:  Mol Biotechnol       Date:  2006-02       Impact factor: 2.695

3.  Expression of the rotavirus SA11 protein VP7 in the simple eukaryote Dictyostelium discoideum.

Authors:  K R Emslie; J M Miller; M B Slade; P R Dormitzer; H B Greenberg; K L Williams
Journal:  J Virol       Date:  1995-03       Impact factor: 5.103

4.  Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.

Authors:  Mahmoud Soliman; Ja-Young Seo; Deok-Song Kim; Ji-Yun Kim; Jun-Gyu Park; Mia Madel Alfajaro; Yeong-Bin Baek; Eun-Hyo Cho; Joseph Kwon; Jong-Soon Choi; Mun-Il Kang; Sang-Ik Park; Kyoung-Oh Cho
Journal:  PLoS Pathog       Date:  2018-01-19       Impact factor: 6.823

5.  Transmissible gastroenteritis virus (TGEV)-based vectors with engineered murine tropism express the rotavirus VP7 protein and immunize mice against rotavirus.

Authors:  Juan Manuel Ribes; Javier Ortego; Juan Ceriani; Rebeca Montava; Luis Enjuanes; Javier Buesa
Journal:  Virology       Date:  2010-11-21       Impact factor: 3.616

6.  Recombinant outer capsid glycoprotein (VP7) of rotavirus expressed in insect cells induces neutralizing antibodies in rabbits.

Authors:  M Khodabandehloo; M Shamsi Shahrabadi; H Keyvani; B Bambai; Za Sadigh
Journal:  Iran J Public Health       Date:  2012-05-31       Impact factor: 1.429

  6 in total

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