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Literature DB >> 1317838

Gene transfer in primary cultures of human hepatocytes.

Abstract

Using liposomes as the mediator of DNA transfer, we were successful in the transfection of human hepatocytes isolated from surgical samples with an E. coli beta-galactosidase gene (beta-gal). A comparison of transfection efficiency showed that of the four promoters used, cytomegalovirus (CMV) promoter yielded higher transfection efficiencies than Rous sarcoma virus (RSV), Simian virus-40 (SV-40) and human alpha-1 antitrypsin (AAT) promoters. These studies represent the first report on the successful transfection of primary cultures of human hepatocytes.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1992 PMID： 1317838 DOI： 10.1007/bf02877062

Source DB: PubMed Journal: In Vitro Cell Dev Biol ISSN： 0883-8364

17 in total

Review 1. Delivery systems for gene therapy.

Authors: G Y Wu; C H Wu
Journal: Biotherapy Date: 1991

2. Evaluation of relative promoter strength in primary hepatocytes using optimized lipofection.

Authors: K P Ponder; R P Dunbar; D R Wilson; G J Darlington; S L Woo
Journal: Hum Gene Ther Date: 1991 Impact factor: 5.695

3. High efficiency gene transfection by electroporation using a radio-frequency electric field.

Authors: D C Chang; P Q Gao; B L Maxwell
Journal: Biochim Biophys Acta Date: 1991-04-17

4. Maintenance of differentiated function in cultured rat hepatocytes immortalized by transfection with viral DNA.

Authors: A Nairn; B Willett; M H Grant; A Scott; C MacDonald
Journal: Biochem Soc Trans Date: 1990-12 Impact factor: 5.407

5. Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression of the bacterial gene.

Authors: G R MacGregor; A E Mogg; J F Burke; C T Caskey
Journal: Somat Cell Mol Genet Date: 1987-05

6. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells.

Authors: G R MacGregor; C T Caskey
Journal: Nucleic Acids Res Date: 1989-03-25 Impact factor: 16.971

7. Electroporation of cultured adult rat hepatocytes with the c-myc gene potentiates DNA synthesis in response to epidermal growth factor.

Authors: S F Muakkassah-Kelly; D A Jans; N Lydon; F Bieri; F Waechter; P Bentley; W Stäubli
Journal: Exp Cell Res Date: 1988-10 Impact factor: 3.905

5. Transcriptional regulation of stromelysin-1 gene expression is altered during progression of mouse mammary epithelial cells from functionally normal to malignant.

Authors: A Lochter; Z Werb; M J Bissell
Journal: Matrix Biol Date: 1999-10 Impact factor: 11.583

6. alpha1 and alpha2 integrins mediate invasive activity of mouse mammary carcinoma cells through regulation of stromelysin-1 expression.

Authors: A Lochter; M Navre; Z Werb; M J Bissell
Journal: Mol Biol Cell Date: 1999-02 Impact factor: 4.138

6 in total

Gene transfer in primary cultures of human hepatocytes.

Review 1. Delivery systems for gene therapy.

2. Evaluation of relative promoter strength in primary hepatocytes using optimized lipofection.

3. High efficiency gene transfection by electroporation using a radio-frequency electric field.

4. Maintenance of differentiated function in cultured rat hepatocytes immortalized by transfection with viral DNA.

5. Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression of the bacterial gene.

6. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells.

7. Electroporation of cultured adult rat hepatocytes with the c-myc gene potentiates DNA synthesis in response to epidermal growth factor.

8. Efficient DNA-mediated gene transfer into primary cultures of adult rat hepatocytes.

9. Promutagen activation by freshly isolated and cryopreserved rat hepatocytes.

10. Transformation of differentiated rat hepatocytes with adenovirus and adenovirus DNA.

1. A simplified method for the culturing of primary adult rat and human hepatocytes as multicellular spheroids.

2. Glyco-poly-l-lysine is better than liposomal delivery of exogenous genes to rat of liver.

3. Role of protein kinase A in human hepatocyte DNA synthesis.

4. Culturing of primary hepatocytes as entrapped aggregates in a packed bed bioreactor: a potential bioartificial liver.

5. Transcriptional regulation of stromelysin-1 gene expression is altered during progression of mouse mammary epithelial cells from functionally normal to malignant.

6. alpha1 and alpha2 integrins mediate invasive activity of mouse mammary carcinoma cells through regulation of stromelysin-1 expression.