| Literature DB >> 1314702 |
M P Peppelenbosch1, L G Tertoolen, J den Hertog, S W de Laat.
Abstract
Epidermal growth factor (EGF) induces a Ca2+ influx in many cell types, but the underlying mechanisms are so far unresolved. We report that: EGF-induced Ca2+ channel activity is eliminated by lipoxygenase inhibition and is mimicked by artificial induction of lipoxygenase activity; addition of leukotriene C4 can fully mimic EGF in its ability to activate Ca2+ channels; and EGF induces a rapid accumulation of intracellular leukotriene C4. In addition, we show that EGF-induced, Ca(2+)-dependent membrane hyperpolarization and junB proto-oncogene expression are dependent on lipoxygenase activity, whereas EGF-induced cytoplasmic alkalinization is not. We conclude that PLA2/5-lipoxygenase-mediated leukotriene C4 production constitutes a novel and specific signal transduction pathway in growth factor action.Entities:
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Year: 1992 PMID： 1314702 DOI： 10.1016/0092-8674(92)90410-e
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582