Literature DB >> 1314582

Na+/H+ exchange in porcine cerebral capillary endothelial cells is inhibited by a benzoylguanidine derivative.

A Schmid1, W Scholz, H J Lang, R Popp.   

Abstract

Na+/H+ exchange activity has been examined in endothelial cells isolated from porcine brain capillaries. Intracellular pH (pHi) changes were monitored using a confocal laser scanning microscope and the pH-sensitive fluorescence indicator 2',7'-bis-(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF). Acid load of the brain capillary endothelial cells was performed with a NH4Cl (20 mM) prepulse. In bicarbonate-free solutions pHi recovered within 3 to 10 min. Removal of extracellular Na+ ions demonstrated that H+ extrusion after an acid load of the cells was Na+ dependent. The Na+/H+ exchange could be completely blocked by EIPA (5-(N-ethyl-N-isopropyl)amiloride) as well as by the novel inhibitor 3-methylsulfonyl-4-piperidinobenzoyl guanidine hydrochloride (HOE 694) in concentrations of 1 to 10 microM, respectively. EIPA and HOE 694 in a concentration of 0.1 microM caused a partial block of Na+/H+ exchange.

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Year:  1992        PMID： 1314582     DOI： 10.1016/0006-291x(92)91165-m

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

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2.  Epithelial myosin light chain kinase-dependent barrier dysfunction mediates T cell activation-induced diarrhea in vivo.

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3.  Cell shrinkage stimulates bradykinin-induced cell membrane potential oscillations in NIH 3T3 fibroblasts expressing the ras-oncogene.

Authors:  M Ritter; E Wöll; S Waldegger; D Häussinger; H J Lang; W Scholz; B Schölkens; F Lang
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4.  Hoe 694, a new Na+/H+ exchange inhibitor and its effects in cardiac ischaemia.

Authors:  W Scholz; U Albus; H J Lang; W Linz; P A Martorana; H C Englert; B A Schölkens
Journal:  Br J Pharmacol       Date:  1993-06       Impact factor: 8.739

5.  A halocin acting on Na+/H+ exchanger of haloarchaea as a new type of inhibitor in NHE of mammals.

Authors:  J L Lequerica; J E O'Connor; L Such; A Alberola; I Meseguer; M Dolz; M Torreblanca; A Moya; F Colom; B Soria
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6.  Inhibition of Na+/H+ exchange preserves viability, restores mechanical function, and prevents the pH paradox in reperfusion injury to rat neonatal myocytes.

Authors:  I S Harper; J M Bond; E Chacon; J M Reece; B Herman; J J Lemasters
Journal:  Basic Res Cardiol       Date:  1993 Sep-Oct       Impact factor: 17.165

Review 7.  Endothelial metabolism in pulmonary vascular homeostasis and acute respiratory distress syndrome.

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Journal:  Am J Physiol Lung Cell Mol Physiol       Date:  2021-06-23       Impact factor: 6.011
  7 in total

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