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Literature DB >> 1313965

Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes.

B Clarke¹, P Stancombe, T Money, T Foote, G Moore.

Abstract

We describe here a protocol for obtaining clones containing sequences present in low copy-number from genomic DNA where moderately and highly repeated sequences predominate. Specific chromosomal regions can be targeted by using deletion or addition line material. We have used this protocol to identify a sequence which has been deleted in both the tetraploid and hexaploid wheat mutants for the homoeologous chromosome pairing locus.

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Year: 1992 PMID： 1313965 PMCID： PMC312172 DOI： 10.1093/nar/20.6.1289

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

6 in total

1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

2. Room temperature method for increasing the rate of DNA reassociation by many thousandfold: the phenol emulsion reassociation technique.

Authors: D E Kohne; S A Levison; M J Byers
Journal: Biochemistry Date: 1977-11-29 Impact factor: 3.162

3. Genome size and the proportion of repeated nucleotide sequence DNA in plants.

Authors: R B Flavell; M D Bennett; J B Smith; D B Smith
Journal: Biochem Genet Date: 1974-10 Impact factor: 1.890

4. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

Authors: R J Britten; D E Kohne
Journal: Science Date: 1968-08-09 Impact factor: 47.728

5. Sequence organisation analysis of the wheat and rye genomes by interspecies DNA/DNA hybridisation.

Authors: J Rimpau; D Smith; R Flavell
Journal: J Mol Biol Date: 1978-08-15 Impact factor: 5.469

6. Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion.

Authors: L M Kunkel; A P Monaco; W Middlesworth; H D Ochs; S A Latt
Journal: Proc Natl Acad Sci U S A Date: 1985-07 Impact factor: 11.205

6 in total

11 in total

Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes.

1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

2. Room temperature method for increasing the rate of DNA reassociation by many thousandfold: the phenol emulsion reassociation technique.

3. Genome size and the proportion of repeated nucleotide sequence DNA in plants.

4. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

5. Sequence organisation analysis of the wheat and rye genomes by interspecies DNA/DNA hybridisation.

6. Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion.

1. Extended genetic maps of the homoeologous group 3 chromosomes of wheat, rye and barley.

2. Chromosomal rearrangements in the rye genome relative to that of wheat.

3. RFLP mapping of genes affecting plant height and growth habit in rye.

4. AFLP-based mRNA fingerprinting.

5. Rapid reorganization of resistance gene homologues in cereal genomes.

6. Detailed comparative mapping of cereal chromosome regions corresponding to the Ph1 locus in wheat.

7. Oat-maize chromosome addition lines: a new system for mapping the maize genome.

8. RFLP-based genetic maps of the homoeologous group 5 chromosomes of bread wheat (Triticum aestivum L.).

9. Isolation and characterization of genome-specific DNA sequences in Triticeae species.

10. Construction of a chromosome-enriched HpaII library from flow-sorted wheat chromosomes.