| Literature DB >> 13130080 |
Steven P Suchyta1, Sue Sipkovsky, Rachael Kruska, Abra Jeffers, Amanda McNulty, Matthew J Coussens, Robert J Tempelman, Robert G Halgren, Peter M Saama, Dale E Bauman, Yves R Boisclair, Jeanne L Burton, Robert J Collier, Edward J DePeters, Theodore A Ferris, Matthew C Lucy, Mark A McGuire, Juan F Medrano, Thomas R Overton, Timothy P Smith, George W Smith, Tad S Sonstegard, James N Spain, Donald E Spiers, Jianbo Yao, Paul M Coussens.
Abstract
A cDNA microarray resource has been developed with the goal of providing integrated functional genomics resources for cattle. The National Bovine Functional Genomics Consortium's (NBFGC) expressed sequence tag (EST) collection was established in 2001 to develop resources for functional genomics research. The NBFGC EST collection and microarray contains 18,263 unique transcripts, derived from many different tissue types and various physiologically important states within these tissues. The NBFGC microarray has been tested for false-positive rates using self-self hybridizations and was shown to yield robust results in test microarray experiments. A web-accessible database has been established to provide pertinent data related to NBFGC clones, including sequence data, BLAST results, and ontology information. The NBFGC microarray represents the largest cDNA microarray for a livestock species prepared to date and should prove to be a valuable tool in studying genome-wide gene expression in cattle.Entities:
Mesh：
Year: 2003 PMID： 13130080 DOI： 10.1152/physiolgenomics.00094.2003
Source DB: PubMed Journal: Physiol Genomics ISSN： 1094-8341 Impact factor: 3.107