Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval 'Virgilkapelle' in Vienna.

Two airborne bacterial isolates designated V45(T) and V54A(T) were characterized in order to determine their taxonomic position. 16S rDNA sequence analysis showed that the two isolates shared 98.1 % sequence similarity. Highest sequence similarities (98.0-98.5 %) were found to Promicromonospora citrea DSM 43110(T) and Promicromonospora sukumoe IFO 14650(T). Detection of a quinone system with the predominant compound MK-9(H(4)), a polar lipid pattern containing phosphatidylglycerol, a fatty acid profile with the predominant acids C(15 : 0) iso and C(15 : 0) anteiso and the diagnostic cell-wall diamino acid L-lysine supported the assignment of the novel isolates to the genus PROMICROMONOSPORA: The two isolates could be distinguished from P. sukumoe by the presence of glycine in the peptidoglycan, and the detection of the cell-wall sugar galactose differentiates them from the two established species of the genus PROMICROMONOSPORA: Each of the two isolates displayed a unique biochemical profile. Results from DNA-DNA hybridizations clearly demonstrated that V45(T) and V54A(T) represent separate species. Based on these data, it is proposed that V45(T) (=IFO 16525(T)=CCM 7044(T)) and V54A(T) (=IFO 16526(T) =CCM 7043(T)) be classified as the type strains of two novel Promicromonospora species, for which the names Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov. are proposed.