Characteristics and function of Ca(2+)- and inositol 1,4,5-trisphosphate-releasable stores of Ca2+ in neurons.

Molecular, biochemical and physiological evidence for the existence of releasable Ca2+ stores in neurons is strong. There are two separate molecules that function as release channels from those Ca2+ stores, the RyanR and InsP3R, and both have multiple regulatory sites for positive and negative control. Perhaps most intriguing is the biphasic, concentration-dependent action of cytosolic Ca2+ on both channels, first to stimulate release then, at higher concentration, to depress release. Whether the InsP3R and RyanR channels regulate Ca2+ release from different or identical functional compartments will need to be defined for each neuron type and perhaps even for each intracellular region within neurons since the evidence for functional separation of stores is mixed. The identification of Ca2+ storage and releasing capacity throughout all subcellular regions of neurons and the increasing evidence for a role for Ca2+ stores in neuronal plasticity suggests that the further characterization of the functional properties of Ca2+ stores will be an increasingly important and expanding area of interest in neurobiology.