| Literature DB >> 1311258 |
I Dornreiter1, L F Erdile, I U Gilbert, D von Winkler, T J Kelly, E Fanning.
Abstract
The purified human single-stranded DNA binding protein, replication protein A (RP-A), forms specific complexes with purified SV40 large T antigen and with purified DNA polymerase alpha-primase, as shown by ELISA and a modified immunoblotting technique. RP-A associated efficiently with the isolated primase, as well as with intact polymerase alpha-primase. The 70 kDa subunit of RP-A was sufficient for association with polymerase alpha-primase. Purified SV40 large T antigen bound to intact RP-A and to polymerase-primase, but not to any of the separated subunits of RP-A or to the isolated primase. These results suggest that the specific protein-protein interactions between RP-A, polymerase-primase and T antigen may play a role in the initiating of SV40 DNA replication.Entities:
Mesh:
Substances:
Year: 1992 PMID: 1311258 PMCID: PMC556510 DOI: 10.1002/j.1460-2075.1992.tb05110.x
Source DB: PubMed Journal: EMBO J ISSN: 0261-4189 Impact factor: 11.598