| Literature DB >> 1311136 |
H Ghiasi1, R Kaiwar, A B Nesburn, S L Wechsler.
Abstract
A recombinant baculovirus (vAc-gB1) was constructed which expresses the glycoprotein B (gB) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with these recombinant viruses, a protein that was close in size to authentic HSV-1 gB was detected by gB polyclonal antibody. The recombinant gB was found on the membrane of Sf9 cells and was susceptible to tunicamycin, glycosidase F (PNGase F) and partially susceptible to Endo-H. Antibodies raised in mice to this recombinant recognized viral gB and neutralized the infectivity of HSV-1 in vitro. Mice inoculated with the recombinant gB were protected from lethal challenge with HSV-1.Entities:
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Year: 1992 PMID： 1311136 DOI： 10.1016/0168-1702(92)90087-p
Source DB: PubMed Journal: Virus Res ISSN： 0168-1702 Impact factor: 3.303