Literature DB >> 1310782

EBNA1 distorts oriP, the Epstein-Barr virus latent replication origin.

L Frappier1, M O'Donnell.   

Abstract

The Epstein-Barr virus nuclear antigen 1 (EBNA1) protein binds and activates the latent replication origin (oriP) of the Epstein-Barr virus. We have been studying EBNA1 to determine how it activates replication at oriP. Here we demonstrate that upon binding of EBNA1 to oriP, two thymine residues become reactive to potassium permanganate (KMnO4), indicating a helical distortion at these sites. The KMnO4-reactive thymines are 64 bp apart in the region of dyad symmetry of oriP. Dimethyl sulfate protection studies indicated that EBNA1 binds on the opposite face of the helix from the reactive thymines. The nature of the helical distortion induced by EBNA1 and its possible significance to the initiation of replication are discussed.

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Year:  1992        PMID： 1310782      PMCID： PMC240939     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  34 in total

1.  Epstein-Barr nuclear antigen 1 mediates a DNA loop within the latent replication origin of Epstein-Barr virus.

Authors:  L Frappier; M O'Donnell
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205

2.  The Epstein-Barr virus origin of plasmid replication, oriP, contains both the initiation and termination sites of DNA replication.

Authors:  T A Gahn; C L Schildkraut
Journal:  Cell       Date:  1989-08-11       Impact factor: 41.582

3.  Conformational changes in a replication origin induced by an initiator protein.

Authors:  S Mukherjee; I Patel; D Bastia
Journal:  Cell       Date:  1985-11       Impact factor: 41.582

4.  Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.

Authors:  M Dodson; H Echols; S Wickner; C Alfano; K Mensa-Wilmot; B Gomes; J LeBowitz; J D Roberts; R McMacken
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

5.  KMnO4 as a probe for lac promoter DNA melting and mechanism in vivo.

Authors:  S Sasse-Dwight; J D Gralla
Journal:  J Biol Chem       Date:  1989-05-15       Impact factor: 5.157

6.  RNA polymerase unwinds an 11-base pair segment of a phage T7 promoter.

Authors:  U Siebenlist
Journal:  Nature       Date:  1979-06-14       Impact factor: 49.962

7.  High-resolution analysis of lac transcription complexes inside cells.

Authors:  J A Borowiec; J D Gralla
Journal:  Biochemistry       Date:  1986-09-09       Impact factor: 3.162

8.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

9.  A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus.

Authors:  B Sugden; K Marsh; J Yates
Journal:  Mol Cell Biol       Date:  1985-02       Impact factor: 4.272

10.  Possible role for thymine glycol in the selective inhibition of DNA synthesis on oxidized DNA templates.

Authors:  P Rouet; J M Essigmann
Journal:  Cancer Res       Date:  1985-12       Impact factor: 12.701
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  27 in total

1.  Strong minor groove base conservation in sequence logos implies DNA distortion or base flipping during replication and transcription initiation.

Authors:  T D Schneider
Journal:  Nucleic Acids Res       Date:  2001-12-01       Impact factor: 16.971

2.  Epstein-Barr nuclear antigen 1 binds and destabilizes nucleosomes at the viral origin of latent DNA replication.

Authors:  T M Avolio-Hunter; P N Lewis; L Frappier
Journal:  Nucleic Acids Res       Date:  2001-09-01       Impact factor: 16.971

3.  The replicator of the Epstein-Barr virus latent cycle origin of DNA replication, oriP, is composed of multiple functional elements.

Authors:  M D Koons; S Van Scoy; J Hearing
Journal:  J Virol       Date:  2001-11       Impact factor: 5.103

4.  The DnaC helicase loader is a dual ATP/ADP switch protein.

Authors:  Megan J Davey; Linhua Fang; Peter McInerney; Roxana E Georgescu; Mike O'Donnell
Journal:  EMBO J       Date:  2002-06-17       Impact factor: 11.598

5.  KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.

Authors:  Rajesh Ponnusamy; Maxim V Petoukhov; Bruno Correia; Tania F Custodio; Franceline Juillard; Min Tan; Marta Pires de Miranda; Maria A Carrondo; J Pedro Simas; Kenneth M Kaye; Dmitri I Svergun; Colin E McVey
Journal:  Nucleic Acids Res       Date:  2015-09-30       Impact factor: 16.971

6.  Epstein-Barr nuclear antigen 1 (EBNA1)-dependent recruitment of origin recognition complex (Orc) on oriP of Epstein-Barr virus with purified proteins: stimulation by Cdc6 through its direct interaction with EBNA1.

Authors:  Kenji Moriyama; Naoko Yoshizawa-Sugata; Chikashi Obuse; Toshiki Tsurimoto; Hisao Masai
Journal:  J Biol Chem       Date:  2012-05-14       Impact factor: 5.157

7.  Functional and physical interactions between the Epstein-Barr virus (EBV) proteins BZLF1 and BMRF1: Effects on EBV transcription and lytic replication.

Authors:  Q Zhang; Y Hong; D Dorsky; E Holley-Guthrie; S Zalani; N A Elshiekh; A Kiehl; T Le; S Kenney
Journal:  J Virol       Date:  1996-08       Impact factor: 5.103

8.  Rep*: a viral element that can partially replace the origin of plasmid DNA synthesis of Epstein-Barr virus.

Authors:  A L Kirchmaier; B Sugden
Journal:  J Virol       Date:  1998-06       Impact factor: 5.103

Review 9.  Molecular virology of Epstein-Barr virus.

Authors:  G W Bornkamm; W Hammerschmidt
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2001-04-29       Impact factor: 6.237

10.  A multifunctional plasmid-encoded replication initiation protein both recruits and positions an active helicase at the replication origin.

Authors:  Yong Jiang; Marcin Pacek; Donald R Helinski; Igor Konieczny; Aresa Toukdarian
Journal:  Proc Natl Acad Sci U S A       Date:  2003-06-30       Impact factor: 11.205
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