Literature DB >> 1309521

A directional, high-frequency chromosomal mobilization system for genetic mapping of Rhizobium meliloti.

S Klein1, K Lohman, R Clover, G C Walker, E R Signer.   

Abstract

A system for mapping of the Rhizobium meliloti chromosome that utilizes transposon Tn5-Mob, which carries the mobilization site of IncP plasmid RP4 (R. Simon, Mol. Gen. Genet. 196:413-420, 1984), was developed. Insertions of Tn5-Mob that were located at particular sites on the R. meliloti chromosome were isolated and served as origins of high-frequency chromosomal transfer when IncP tra functions were provided in trans. This approach is, in principle, applicable to any gram-negative bacterium in which Tn5 can transpose and into which IncP plasmids can conjugate.

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Year:  1992        PMID: 1309521      PMCID: PMC205713          DOI: 10.1128/jb.174.1.324-326.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  13 in total

1.  Genetic manipulations in Rhizobium meliloti utilizing two new transposon Tn5 derivatives.

Authors:  G F De Vos; G C Walker; E R Signer
Journal:  Mol Gen Genet       Date:  1986-09

2.  Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes.

Authors:  T M Finan; B Kunkel; G F De Vos; E R Signer
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

3.  A novel exopolysaccharide can function in place of the calcofluor-binding exopolysaccharide in nodulation of alfalfa by Rhizobium meliloti.

Authors:  J Glazebrook; G C Walker
Journal:  Cell       Date:  1989-02-24       Impact factor: 41.582

4.  Genetic map of Rhizobium meliloti megaplasmid pRmeSU47b.

Authors:  T C Charles; T M Finan
Journal:  J Bacteriol       Date:  1990-05       Impact factor: 3.490

5.  Genetic analysis of a cluster of genes required for synthesis of the calcofluor-binding exopolysaccharide of Rhizobium meliloti.

Authors:  S Long; J W Reed; J Himawan; G C Walker
Journal:  J Bacteriol       Date:  1988-09       Impact factor: 3.490

6.  High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn5-Mob transposon.

Authors:  R Simon
Journal:  Mol Gen Genet       Date:  1984

7.  Conjugal transfer of bacterial chromosomes mediated by the RK2 plasmid transfer origin cloned into transposon Tn5.

Authors:  E A Yakobson; D G Guiney
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

8.  Generalized transduction in Rhizobium meliloti.

Authors:  M O Martin; S R Long
Journal:  J Bacteriol       Date:  1984-07       Impact factor: 3.490

9.  General transduction in Rhizobium meliloti.

Authors:  T M Finan; E Hartweig; K LeMieux; K Bergman; G C Walker; E R Signer
Journal:  J Bacteriol       Date:  1984-07       Impact factor: 3.490

10.  Genetic mapping of Rhizobium meliloti.

Authors:  H M Meade; E R Signer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205

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  7 in total

1.  Genetic characterization of a Sinorhizobium meliloti chromosomal region in lipopolysaccharide biosynthesis.

Authors:  A Lagares; D F Hozbor; K Niehaus; A J Otero; J Lorenzen; W Arnold; A Pühler
Journal:  J Bacteriol       Date:  2001-02       Impact factor: 3.490

2.  Regulation of phosphate assimilation in Rhizobium (Sinorhizobium) meliloti.

Authors:  S D Bardin; T M Finan
Journal:  Genetics       Date:  1998-04       Impact factor: 4.562

3.  A high-density physical map of Sinorhizobium meliloti 1021 chromosome derived from bacterial artificial chromosome library.

Authors:  D Capela; F Barloy-Hubler; M T Gatius; J Gouzy; F Galibert
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

4.  Identification of an acetoacetyl coenzyme A synthetase-dependent pathway for utilization of L-(+)-3-hydroxybutyrate in Sinorhizobium meliloti.

Authors:  Punita Aneja; Renata Dziak; Guo-Qin Cai; Trevor C Charles
Journal:  J Bacteriol       Date:  2002-03       Impact factor: 3.490

5.  Second site mutations specifically suppress the Fix- phenotype of Rhizobium meliloti ndvF mutations on alfalfa: identification of a conditional ndvF-dependent mucoid colony phenotype.

Authors:  I J Oresnik; T C Charles; T M Finan
Journal:  Genetics       Date:  1994-04       Impact factor: 4.562

6.  Rhizobium meliloti contains a novel second homolog of the cell division gene ftsZ.

Authors:  W Margolin; S R Long
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

7.  Correlated physical and genetic map of the Bradyrhizobium japonicum 110 genome.

Authors:  C Kündig; H Hennecke; M Göttfert
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

  7 in total

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