Literature DB >> 1307410

Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparison of the assay with ELISA.

E D Camargo1, P M Nakamura, A J Vaz, M V da Silva, P P Chieffi, E O de Melo.   

Abstract

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1307410     DOI： 10.1590/s0036-46651992000100010

Source DB:  PubMed          Journal:  Rev Inst Med Trop Sao Paulo        ISSN： 0036-4665            Impact factor:   1.846


  7 in total

1.  A Lateral Flow Rapid Test for Human Toxocariasis Developed Using Three Toxocara canis Recombinant Antigens.

Authors:  Muhammad Hafiznur Yunus; Siti Naqiuyah Tan Farrizam; Izzati Zahidah Abdul Karim; Rahmah Noordin
Journal:  Am J Trop Med Hyg       Date:  2018-01-01       Impact factor: 2.345

2.  Toxocara spp. seroprevalence in sheep from southern Brazil.

Authors:  Gabriela Lopes Rassier; Sibele Borsuk; Felipe Pappen; Carlos Jaime Scaini; Tiago Gallina; Marcos Marreiro Villela; Nara Amélia da Rosa Farias; Magda Vieira Benavides; Maria Elisabeth Aires Berne
Journal:  Parasitol Res       Date:  2013-07-07       Impact factor: 2.289

3.  Dot enzyme-linked immunosorbent assay (ELISA) for the detection of Toxocara infection using a rat model.

Authors:  Vachel Gay V Paller; Cyrelle M Besana; Isabel Kristine M Valdez
Journal:  J Parasit Dis       Date:  2017-04-05

Review 4.  Update on eosinophilic meningoencephalitis and its clinical relevance.

Authors:  Carlos Graeff-Teixeira; Ana Cristina Arámburu da Silva; Kentaro Yoshimura
Journal:  Clin Microbiol Rev       Date:  2009-04       Impact factor: 26.132

5.  The Seropositivity of Toxocara spp. Antibodies in Pregnant Women Attented at the University Hospital in Southern Brazil and the Factors Associated with Infection.

Authors:  Paula Costa Santos; Lis Maurente Lehmann; Carolina Lorenzi; Carolina Hirsch; Paula Lima Telmo; Gabriela Torres Mattos; Priscila Silva Cadore; Gabriel Baracy Klafke; Maria Elisabeth Aires Berne; Carla Vitola Gonçalves; Carlos James Scaini
Journal:  PLoS One       Date:  2015-07-06       Impact factor: 3.240

6.  Seropositivity for ascariosis and toxocariosis and cytokine expression among the indigenous people in the Venezuelan Delta region.

Authors:  Zaida Araujo; Sietze Brandes; Elena Pinelli; María A Bochichio; Andrea Palacios; Albina Wide; Bruno Rivas-Santiago; Juan Carlos Jiménez
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2015 Jan-Feb       Impact factor: 1.846

7.  Seroprevalence and modifiable risk factors for Toxocara spp. in Brazilian schoolchildren.

Authors:  Alex J F Cassenote; Alba R de Abreu Lima; José M Pinto Neto; Guita Rubinsky-Elefant
Journal:  PLoS Negl Trop Dis       Date:  2014-05-29
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.