Literature DB >> 1303803

Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs.

M Minet1, M E Dufour, F Lacroute.   

Abstract

An Arabidopsis thaliana cDNA bank has been constituted in a Saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (PGK) promoter and terminator. This bank was used to complement eight S. cerevisiae auxotrophic markers. All of them were corrected. These results confirm the quality of the bank and the feasibility of cloning plant genes by yeast mutant complementation. The cDNA complementing the ura1 yeast mutant was sequenced, analysed and shown to encode a dihydroorotic (DHO) dehydrogenase sequence.

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Year:  1992        PMID: 1303803     DOI: 10.1111/j.1365-313x.1992.00417.x

Source DB:  PubMed          Journal:  Plant J        ISSN: 0960-7412            Impact factor:   6.417


  202 in total

1.  Amino acid transporters are localized to transfer cells of developing pea seeds.

Authors:  M Tegeder; C E Offler; W B Frommer; J W Patrick
Journal:  Plant Physiol       Date:  2000-02       Impact factor: 8.340

2.  Methods of double-stranded RNA-mediated gene inactivation in Arabidopsis and their use to define an essential gene in methionine biosynthesis.

Authors:  J Z Levin; A J de Framond; A Tuttle; M W Bauer; P B Heifetz
Journal:  Plant Mol Biol       Date:  2000-12       Impact factor: 4.076

3.  Altered selectivity in an Arabidopsis metal transporter.

Authors:  E E Rogers; D J Eide; M L Guerinot
Journal:  Proc Natl Acad Sci U S A       Date:  2000-10-24       Impact factor: 11.205

4.  AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides.

Authors:  Francisco de La Torre; Javier Sampedro; Ignacio Zarra; Gloria Revilla
Journal:  Plant Physiol       Date:  2002-01       Impact factor: 8.340

5.  Characterization of CAX4, an Arabidopsis H(+)/cation antiporter.

Authors:  Ning-hui Cheng; Jon K Pittman; Toshiro Shigaki; Kendal D Hirschi
Journal:  Plant Physiol       Date:  2002-04       Impact factor: 8.340

6.  Two iron-regulated cation transporters from tomato complement metal uptake-deficient yeast mutants.

Authors:  U Eckhardt; A Mas Marques; T J Buckhout
Journal:  Plant Mol Biol       Date:  2001-03       Impact factor: 4.076

7.  At-ACA8 encodes a plasma membrane-localized calcium-ATPase of Arabidopsis with a calmodulin-binding domain at the N terminus.

Authors:  M C Bonza; P Morandini; L Luoni; M Geisler; M G Palmgren; M I De Michelis
Journal:  Plant Physiol       Date:  2000-08       Impact factor: 8.340

8.  AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene.

Authors:  Y P Lu; Z S Li; P A Rea
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205

9.  Human and Saccharomyces cerevisiae dolichol phosphate mannose synthases represent two classes of the enzyme, but both function in Schizosaccharomyces pombe.

Authors:  P A Colussi; C H Taron; J C Mack; P Orlean
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205

10.  Isolation of a cDNA encoding an Arabidopsis galactokinase by functional expression in yeast.

Authors:  C P Kaplan; H B Tugal; A Baker
Journal:  Plant Mol Biol       Date:  1997-06       Impact factor: 4.076

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