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Literature DB >> 1300234

Stable triple helices are formed upon binding of RNA oligonucleotides and their 2'-O-methyl derivatives to double-helical DNA.

C Escudé¹, J S Sun, M Rougée, T Garestier, C Hélène.

Abstract

Pyrimidine oligoribonucleotides bind to the major groove of double-helical DNA at homopurine.homopyrimidine sequences. They recognize Watson-Crick base pairs by forming T.A x U and C.G x C base triplets via Hoogsteen hydrogen bonding. The stability of these triple helices is much higher than that of triple helices formed by oligodeoxyribonucleotides as shown by an increase of the temperature at which half-dissociation of the third strand occurs. When the 2'-hydroxyl group of ribose moieties is replaced by 2'-O-methyl substituent, triple helix stability is further increased.

Entities: Chemical

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Year: 1992 PMID： 1300234

Source DB: PubMed Journal: C R Acad Sci III ISSN： 0764-4469

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Cited

18 in total

Review 1. Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities.

Authors: Taco G Uil; Hidde J Haisma; Marianne G Rots
Journal: Nucleic Acids Res Date: 2003-11-01 Impact factor: 16.971

2. Relative stabilities of triple helices composed of combinations of DNA, RNA and 2'-O-methyl-RNA backbones: chimeric circular oligonucleotides as probes.

Authors: S Wang; E T Kool
Journal: Nucleic Acids Res Date: 1995-04-11 Impact factor: 16.971

3. Structural features and stability of an RNA triple helix in solution.

Authors: J A Holland; D W Hoffman
Journal: Nucleic Acids Res Date: 1996-07-15 Impact factor: 16.971

4. Stabilization of triple-stranded oligonucleotide complexes: use of probes containing alternating phosphodiester and stereo-uniform cationic phosphoramidate linkages.

Authors: S Chaturvedi; T Horn; R L Letsinger
Journal: Nucleic Acids Res Date: 1996-06-15 Impact factor: 16.971

5. Triplex formation with alpha anomers of purine-rich and pyrimidine-rich oligodeoxynucleotides.

Authors: S B Noonberg; J C François; D Praseuth; A L Guieysse-Peugeot; J Lacoste; T Garestier; C Hélène
Journal: Nucleic Acids Res Date: 1995-10-25 Impact factor: 16.971

6. Preparation and application of triple helix forming oligonucleotides and single strand oligonucleotide donors for gene correction.

Authors: Rowshon Alam; Arun Kalliat Thazhathveetil; Hong Li; Michael M Seidman
Journal: Methods Mol Biol Date: 2014

Stable triple helices are formed upon binding of RNA oligonucleotides and their 2'-O-methyl derivatives to double-helical DNA.

Review 1. Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities.

2. Relative stabilities of triple helices composed of combinations of DNA, RNA and 2'-O-methyl-RNA backbones: chimeric circular oligonucleotides as probes.

3. Structural features and stability of an RNA triple helix in solution.

4. Stabilization of triple-stranded oligonucleotide complexes: use of probes containing alternating phosphodiester and stereo-uniform cationic phosphoramidate linkages.

5. Triplex formation with alpha anomers of purine-rich and pyrimidine-rich oligodeoxynucleotides.

6. Preparation and application of triple helix forming oligonucleotides and single strand oligonucleotide donors for gene correction.

7. Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases.

Review 8. Bioconjugation of oligonucleotides for treating liver fibrosis.

9. Oligonucleotide clamps arrest DNA synthesis on a single-stranded DNA target.

10. Inhibition of transcription by platinated triplex-forming oligonucleotides.