Liquid chromatographic method for separation and determination of elaiophylin in biotechnology processes of salinomycin production.

An LC method for the quick and precise quantitative determination of elaiophylin in biotechnology processes of salinomycin production was prepared. A mobile phase of methanol 0.04 M diammonium hydrogen phosphate pH 6.0-ethyl acetate (55:35:10, v/v/v) and an elevated column temperature optimized the chromatography. Separation of elaiophylin was obtained in 4-6 min. Positive identification and peak purity of elaiophylin have been carried out by photodiode array (PDA) detection. An agreement between the PDA spectra of samples and the elaiophylin standard were obtained.