AIM: To assess the decline in Helicobacter pylori antibodies after eradication of infection. METHODS: The H. pylori status was determined at entry (D0) by culture and histology performed on antral biopsies and after eradication treatment at day 42 (day 42) and after 6 months (M6) by the 13C-urea breath test. The EIA kits used to determine the anti-H. pylori antibody titre were HM-CAP (immunoglobulin-G) and PP-CAP (immunoglobulin-A) kits (Enteric Products, Inc.) and Pyloriset EIA-G (Orion Diagnostica). RESULTS:Ninety-three patients were included. For 82 patients who were successfully treated, no kit was sufficiently accurate at D42 to show eradication. The antibody titre decreased for HM-CAP, PP-CAP and Pyloriset EIA-G by a mean of 35.6%, 41.2% and 64.7% between D0 and M6, respectively. According to the cut-off values defined by the manufacturers, 8.5% (PP-CAP, Pyloriset EIA-G) and 9.7% (HM-CAP) of the patients became H. pylori negative at M6. Using a 25% decrease in antibody titre between D0 and M6 as a threshold for H. pylori eradication, specificity was 100% for HM-CAP, 89.9% for Pyloriset EIA-G and 100% for PP-CAP, whereas the sensitivity was 76.8%, 98.8% and 72%, respectively. CONCLUSION: An antibody titre decrease of 25% at M6 was found to be accurate in confirming H. pylori eradication.
RCT Entities:
AIM: To assess the decline in Helicobacter pylori antibodies after eradication of infection. METHODS: The H. pylori status was determined at entry (D0) by culture and histology performed on antral biopsies and after eradication treatment at day 42 (day 42) and after 6 months (M6) by the 13C-urea breath test. The EIA kits used to determine the anti-H. pylori antibody titre were HM-CAP (immunoglobulin-G) and PP-CAP (immunoglobulin-A) kits (Enteric Products, Inc.) and Pyloriset EIA-G (Orion Diagnostica). RESULTS: Ninety-three patients were included. For 82 patients who were successfully treated, no kit was sufficiently accurate at D42 to show eradication. The antibody titre decreased for HM-CAP, PP-CAP and Pyloriset EIA-G by a mean of 35.6%, 41.2% and 64.7% between D0 and M6, respectively. According to the cut-off values defined by the manufacturers, 8.5% (PP-CAP, Pyloriset EIA-G) and 9.7% (HM-CAP) of the patients became H. pylori negative at M6. Using a 25% decrease in antibody titre between D0 and M6 as a threshold for H. pylori eradication, specificity was 100% for HM-CAP, 89.9% for Pyloriset EIA-G and 100% for PP-CAP, whereas the sensitivity was 76.8%, 98.8% and 72%, respectively. CONCLUSION: An antibody titre decrease of 25% at M6 was found to be accurate in confirming H. pylori eradication.
Authors: Michelle L Turk; Laura D Cacioppo; Zhongming Ge; Zeli Shen; Mark T Whary; Nicola Parry; Samuel R Boutin; Hilton J Klein; James G Fox Journal: J Med Microbiol Date: 2012-02-02 Impact factor: 2.472
Authors: Sebastian Rupp; Apostolis Papaefthymiou; Eleftherios Chatzimichael; Stergios A Polyzos; Stefan Spreitzer; Michael Doulberis; Thomas Kuntzen; Jannis Kountouras Journal: Ann Gastroenterol Date: 2022-06-02