Literature DB >> 12967207

Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis.

Anna M Fitzgerald1, Agnieszka M Mudge, Andrew P Gleave, Kim M Plummer.   

Abstract

We report the development of two new transformation systems, polyethylene glycol (PEG)-mediated transformation of protoplasts and Agrobacterium tumefaciens-mediated transformation of mycelium, for the filamentous ascomycete Venturia inaequalis. New binary vectors have been created for the latter. Although transformation was initially achieved using a PEG-mediated method, this was superseded by the A. tumefaciens-mediated approach. The advantages of the latter include: ease of the protocol, no requirement for protoplasts; higher transformation efficiency; and single-site integration. A comparison between the two transformation methods is presented.

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Year:  2003        PMID： 12967207     DOI： 10.1017/s0953756203008086

Source DB:  PubMed          Journal:  Mycol Res        ISSN： 0953-7562


  17 in total

1.  Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans.

Authors:  Saba Hasan; Rana Inder Singh; Sandhu Sardul Singh
Journal:  Indian J Microbiol       Date:  2011-01-25       Impact factor: 2.461

2.  Genetic transformation of extremophilic fungi Acidea extrema and Acidothrix acidophila.

Authors:  Hana Hršelová; Martina Hujslová; Milan Gryndler
Journal:  Folia Microbiol (Praha)       Date:  2015-05-02       Impact factor: 2.099

3.  Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens.

Authors:  Fernanda L S Sebastianes; Paulo T Lacava; Léia C L Fávaro; Maria B C Rodrigues; Welington L Araújo; João L Azevedo; Aline A Pizzirani-Kleiner
Journal:  Curr Genet       Date:  2011-12-31       Impact factor: 3.886

4.  Genetic modification of asexual Epichloë endophytes with the perA gene for peramine biosynthesis.

Authors:  Inoka K Hettiarachchige; Aaron C Elkins; Priyanka Reddy; Ross C Mann; Kathryn M Guthridge; Timothy I Sawbridge; John W Forster; German C Spangenberg
Journal:  Mol Genet Genomics       Date:  2018-11-15       Impact factor: 3.291

5.  Agrobacterium-Mediated Transformation of Diaporthe schini Endophytes Associated with Vitis labrusca L. and Its Antagonistic Activity Against Grapevine Phytopathogens.

Authors:  Aretusa Cristina Felber; Julio Cesar Polonio; Ravely Casarotti Orlandelli; Alessandra Tenório Costa; Eliane Papa Ambrosio-Albuquerque; Renata Assis Castro; Maria Carolina Quecine-Verdi; João Lúcio de Azevedo; João Alencar Pamphile; Káthia Socorro Mathias Mourão
Journal:  Indian J Microbiol       Date:  2019-03-14       Impact factor: 2.461

Review 6.  Agrobacterium-mediated transformation as a tool for functional genomics in fungi.

Authors:  Caroline B Michielse; Paul J J Hooykaas; Cees A M J J van den Hondel; Arthur F J Ram
Journal:  Curr Genet       Date:  2005-05-12       Impact factor: 3.886

7.  Venturia inaequalis: the causal agent of apple scab.

Authors:  Joanna K Bowen; Carl H Mesarich; Vincent G M Bus; Robert M Beresford; Kim M Plummer; Matthew D Templeton
Journal:  Mol Plant Pathol       Date:  2010-08-26       Impact factor: 5.663

8.  Identification of virulence genes in the corn pathogen Colletotrichum graminicola by Agrobacterium tumefaciens-mediated transformation.

Authors:  Steffen Münch; Nancy Ludwig; Daniela S Floss; Janyce A Sugui; Anna M Koszucka; Lars M Voll; Uwe Sonnewald; Holger B Deising
Journal:  Mol Plant Pathol       Date:  2011-01       Impact factor: 5.663

9.  A new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus Aspergillus oryzae using Agrobacterium tumefaciens-mediated transformation.

Authors:  Khuyen Thi Nguyen; Quynh Ngoc Ho; Loc Thi Binh Xuan Do; Linh Thi Dam Mai; Duc-Ngoc Pham; Huyen Thi Thanh Tran; Diep Hong Le; Huy Quang Nguyen; Van-Tuan Tran
Journal:  World J Microbiol Biotechnol       Date:  2017-05-02       Impact factor: 3.312

10.  Reverse genetics for functional genomics of phytopathogenic fungi and oomycetes.

Authors:  Vijai Bhadauria; Sabine Banniza; Yangdou Wei; You-Liang Peng
Journal:  Comp Funct Genomics       Date:  2009-10-07
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