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Literature DB >> 12965936

Serotyping isolates of Anaplasma phagocytophilum by using monoclonal antibodies.

Hisashi Inokuma¹, Philippe Brouqui, J Stephen Dumler, Didier Raoult.

Abstract

Ten mouse monoclonal antibodies (MAbs) that react with Anaplasma phagocytophilum (the human granulocytic ehrlichiosis agent) Webster isolates were developed. Seven different isolates of A. phagocytophilum were subtyped with these MAbs. Western blot analysis revealed that these MAbs reacted mainly with 41- to 46-kDa Msp2 proteins. Six MAbs reacted with all isolates. Four other MAbs reacted with human isolates from Wisconsin, but not with human isolates from New York or with animal isolates. Three different serotypes were identified. These features may lead to the development of other specific MAbs in order to provide tools for antigenic characterization of human isolates of A. phagocytophilum.

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Year: 2003 PMID： 12965936 PMCID： PMC193879 DOI： 10.1128/cdli.10.5.969-972.2003

Source DB: PubMed Journal: Clin Diagn Lab Immunol ISSN： 1071-412X

17 in total

1. Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic ehrlichiosis agent isolates from Northern California.

Authors: J S Chae; J E Foley; J S Dumler; J E Madigan
Journal: J Clin Microbiol Date: 2000-04 Impact factor: 5.948

2. Improved sensitivity of PCR for diagnosis of human granulocytic ehrlichiosis using epank1 genes of Ehrlichia phagocytophila-group ehrlichiae.

Authors: J J Walls; P Caturegli; J S Bakken; K M Asanovich; J S Dumler
Journal: J Clin Microbiol Date: 2000-01 Impact factor: 5.948

3. Antigenic variation of Anaplasma marginale msp2 occurs by combinatorial gene conversion.

Authors: Kelly A Brayton; Guy H Palmer; Anna Lundgren; Jooyoung Yi; Anthony F Barbet
Journal: Mol Microbiol Date: 2002-03 Impact factor: 3.501

4. Characterization of monoclonal antibodies to an immunodominant protein of the etiologic agent of human granulocytic ehrlichiosis.

Authors: M D Ravyn; L J Lamb; R Jemmerson; J L Goodman; R C Johnson
Journal: Am J Trop Med Hyg Date: 1999-07 Impact factor: 2.345

3. Molecular characterization of Msp2/P44 of Anaplasma phagocytophilum isolated from infected patients and Haemaphysalis longicornis in Laizhou Bay, Shandong Province, China.

Authors: Yong Wang; Chuangfu Chen; Lijuan Zhang
Journal: PLoS One Date: 2013-10-22 Impact factor: 3.240

3 in total

Serotyping isolates of Anaplasma phagocytophilum by using monoclonal antibodies.

1. Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic ehrlichiosis agent isolates from Northern California.

2. Improved sensitivity of PCR for diagnosis of human granulocytic ehrlichiosis using epank1 genes of Ehrlichia phagocytophila-group ehrlichiae.

3. Antigenic variation of Anaplasma marginale msp2 occurs by combinatorial gene conversion.

4. Characterization of monoclonal antibodies to an immunodominant protein of the etiologic agent of human granulocytic ehrlichiosis.

5. Sequence analysis of the ank gene of granulocytic ehrlichiae.

6. Genetic variability and stability of Anaplasma phagocytophila msp2 (p44).

7. Transcript heterogeneity of the p44 multigene family in a human granulocytic ehrlichiosis agent transmitted by ticks.

8. Analysis of sequences and loci of p44 homologs expressed by Anaplasma phagocytophila in acutely infected patients.

9. Genotypic and antigenic identification of two new strains of spotted fever group rickettsiae isolated from China.

10. Differential expression of the p44 gene family in the agent of human granulocytic ehrlichiosis.

1. Reinfection with Anaplasma phagocytophilum in BALB/c mice and cross-protection between two sympatric isolates.

Review 2. Opening the black box of Anaplasma phagocytophilum diversity: current situation and future perspectives.

3. Molecular characterization of Msp2/P44 of Anaplasma phagocytophilum isolated from infected patients and Haemaphysalis longicornis in Laizhou Bay, Shandong Province, China.