Literature DB >> 12963352

Dialysis strategies for protein refolding: preparative streptavidin production.

Hans Peter Sørensen1, Hans Uffe Sperling-Petersen, Kim Kusk Mortensen.   

Abstract

We have investigated different dialysis strategies for the refolding of recombinant streptavidin, and present a novel dialysis setup featuring gradual dilution dialysis and continuous protein feeding into a dialysis sack. A denaturing dialysis buffer is exchanged gradually by dilution with refolding buffer and it is demonstrated that the refolding yield can be increased from 45 to 75% by lowering the dilution rate. In addition, continuous feeding of protein to the dialysis sack increases the yield by 5 to 10%. The principle of gradual dilution dialysis is amenable to stringent regulation and we suggest it to be applied for other insoluble protein targets.

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Year:  2003        PMID： 12963352     DOI： 10.1016/s1046-5928(03)00133-5

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  9 in total

1.  Biotin carboxyl carrier protein co-purifies as a contaminant in core-streptavidin preparations.

Authors:  Welson Wen-Shang Wang; Dipankar Das; Mavanur R Suresh
Journal:  Mol Biotechnol       Date:  2005-09       Impact factor: 2.695

2.  An automatic refolding apparatus for preparative-scale protein production.

Authors:  Yanye Feng; Ming Zhang; Linlin Zhang; Ting Zhang; Jianfeng Ding; Yingping Zhuang; Xiaoning Wang; Zhong Yang
Journal:  PLoS One       Date:  2012-09-27       Impact factor: 3.240

3.  CoESPRIT: a library-based construct screening method for identification and expression of soluble protein complexes.

Authors:  Yingfeng An; Patrick Meresse; Philippe J Mas; Darren J Hart
Journal:  PLoS One       Date:  2011-02-22       Impact factor: 3.240

4.  Recombinant streptavidin nanopeptamer anti-immunocomplex assay for noncompetitive detection of small analytes.

Authors:  Mariana Carlomagno; Gabriel Lassabe; Martín Rossotti; Andrés González-Techera; Lucía Vanrell; Gualberto González-Sapienza
Journal:  Anal Chem       Date:  2014-10-10       Impact factor: 6.986

5.  Production of Recombinant Streptavidin and Optimization of Refolding Conditions for Recovery of Biological Activity.

Authors:  Raoufe Modanloo Jouybari; Abdorrahim Sadeghi; Behzad Khansarinejad; Shabnam Sadoogh Abbasian; Hamid Abtahi
Journal:  Rep Biochem Mol Biol       Date:  2018-04

6.  The RNA polymerase factory: a robotic in vitro assembly platform for high-throughput production of recombinant protein complexes.

Authors:  Sven Nottebaum; Lin Tan; Dominika Trzaska; Hannah C Carney; Robert O J Weinzierl
Journal:  Nucleic Acids Res       Date:  2007-11-19       Impact factor: 16.971

7.  Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins.

Authors:  Luis Felipe Vallejo; Ursula Rinas
Journal:  Microb Cell Fact       Date:  2004-09-02       Impact factor: 5.328

8.  High-level expression of functionally active dengue-2 non-structural antigen 1 production in Escherichia coli.

Authors:  S Gowri Sankar; K J Dhanajeyan; R Paramasivan; V Thenmozhi; B K Tyagi; S John Vennison
Journal:  Biomed Res Int       Date:  2013-09-08       Impact factor: 3.411

9.  Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies.

Authors:  Iman Esmaili; Hamid Mir Mohammad Sadeghi; Vajihe Akbari
Journal:  Res Pharm Sci       Date:  2018-10
  9 in total

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