Literature DB >> 12958300

Multiplex PCRs for identification of necrotoxigenic Escherichia coli.

Sigrid Van Bost1, Etienne Jacquemin, Eric Oswald, Jacques Mainil.   

Abstract

Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necrotoxigenic E. coli strains isolated from different animal species.

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Year:  2003        PMID： 12958300      PMCID： PMC193843          DOI： 10.1128/JCM.41.9.4480-4482.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

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