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Literature DB >> 12957573

Detection and enrichment of antigen-specific CD4+ and CD8+ T cells based on cytokine secretion.

Abstract

The Cytokine Secretion Assay is an innovative method for analysing and enriching live cytokine-secreting cells. In this assay, a cytokine affinity matrix is built on the cell plasma membrane, which traps cytokines produced by the cell in response to specific stimuli. The specifically bound cytokine is then detected, and the cells optionally enriched, using fluorochrome-conjugated cytokine-specific antibodies and magnetic microbeads. This method allows extremely detailed phenotyping of live cells and the detection of cytokine responses at very low frequencies. Here, the latest cell staining and separation procedures are reviewed, with particular reference to the best application of the technology and troubleshooting in a variety of different situations.

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Year: 2003 PMID： 12957573 DOI： 10.1016/s1046-2023(03)00125-7

Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608

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Cited

9 in total

1. CD8(+) T-cell immunity against cancer-testis antigens develops following allogeneic stem cell transplantation and reveals a potential mechanism for the graft-versus-leukemia effect.

Authors: Andrew McLarnon; Karen P Piper; Oliver C Goodyear; Julie M Arrazi; Premini Mahendra; Mark Cook; Fiona Clark; Guy Pratt; Charles Craddock; Paul A H Moss
Journal: Haematologica Date: 2010-05-11 Impact factor: 9.941

Review 2. Rapid detection, enrichment and propagation of specific T cell subsets based on cytokine secretion.

Authors: J D M Campbell; A Foerster; V Lasmanowicz; M Niemöller; A Scheffold; M Fahrendorff; G Rauser; M Assenmacher; A Richter
Journal: Clin Exp Immunol Date: 2010-10-21 Impact factor: 4.330

3. Dynamic relationship between IFN-gamma and IL-2 profile of Mycobacterium tuberculosis-specific T cells and antigen load.

Authors: Kerry A Millington; John A Innes; Sarah Hackforth; Timothy S C Hinks; Jonathan J Deeks; Davinder P S Dosanjh; Valerie Guyot-Revol; Rubamalaar Gunatheesan; Paul Klenerman; Ajit Lalvani
Journal: J Immunol Date: 2007-04-15 Impact factor: 5.422

Review 4. Role of Virus-Specific T Cell Therapy for Cytomegalovirus and BK Infections in Kidney Transplant Recipients.

Authors: Sandesh Parajuli; Margaret Jorgenson; Ross O Meyers; Arjang Djamali; Jacques Galipeau
Journal: Kidney360 Date: 2021-03-26

5. Identification and Characterization of Antigen-Specific CD8⁺ T Cells Using Surface-Trapped TNF-α and Single-Cell Sequencing.

Authors: Shaheed Abdulhaqq; Abigail B Ventura; Jason S Reed; Arman A Bashirova; Katherine B Bateman; Eric McDonald; Helen L Wu; Justin M Greene; John B Schell; David Morrow; Karin Wisskirchen; Jeffrey N Martin; Steven G Deeks; Mary Carrington; Ulrike Protzer; Klaus Früh; Scott G Hansen; Louis J Picker; Jonah B Sacha; Benjamin N Bimber
Journal: J Immunol Date: 2021-11-22 Impact factor: 5.426

9. Multiplexed detection and isolation of viable low-frequency cytokine-secreting human B cells using cytokine secretion assay and flow cytometry (CSA-Flow).

Authors: Ayman Rezk; Rui Li; Amit Bar-Or
Journal: Sci Rep Date: 2020-09-09 Impact factor: 4.379

9 in total

Detection and enrichment of antigen-specific CD4+ and CD8+ T cells based on cytokine secretion.

1. CD8(+) T-cell immunity against cancer-testis antigens develops following allogeneic stem cell transplantation and reveals a potential mechanism for the graft-versus-leukemia effect.

Review 2. Rapid detection, enrichment and propagation of specific T cell subsets based on cytokine secretion.

3. Dynamic relationship between IFN-gamma and IL-2 profile of Mycobacterium tuberculosis-specific T cells and antigen load.

Review 4. Role of Virus-Specific T Cell Therapy for Cytomegalovirus and BK Infections in Kidney Transplant Recipients.

5. Identification and Characterization of Antigen-Specific CD8⁺ T Cells Using Surface-Trapped TNF-α and Single-Cell Sequencing.

6. Red blood cell (RBC) suspensions in confined microflows: Pressure-flow relationship.

Review 7. Is It Feasible to Use CMV-Specific T-Cell Adoptive Transfer as Treatment Against Infection in SOT Recipients?

8. High efficiency ex vivo cloning of antigen-specific human effector T cells.

9. Multiplexed detection and isolation of viable low-frequency cytokine-secreting human B cells using cytokine secretion assay and flow cytometry (CSA-Flow).

Detection and enrichment of antigen-specific CD4+ and CD8+ T cells based on cytokine secretion.

1. CD8(+) T-cell immunity against cancer-testis antigens develops following allogeneic stem cell transplantation and reveals a potential mechanism for the graft-versus-leukemia effect.

Review 2. Rapid detection, enrichment and propagation of specific T cell subsets based on cytokine secretion.

3. Dynamic relationship between IFN-gamma and IL-2 profile of Mycobacterium tuberculosis-specific T cells and antigen load.

Review 4. Role of Virus-Specific T Cell Therapy for Cytomegalovirus and BK Infections in Kidney Transplant Recipients.

5. Identification and Characterization of Antigen-Specific CD8+ T Cells Using Surface-Trapped TNF-α and Single-Cell Sequencing.

6. Red blood cell (RBC) suspensions in confined microflows: Pressure-flow relationship.

Review 7. Is It Feasible to Use CMV-Specific T-Cell Adoptive Transfer as Treatment Against Infection in SOT Recipients?

8. High efficiency ex vivo cloning of antigen-specific human effector T cells.

9. Multiplexed detection and isolation of viable low-frequency cytokine-secreting human B cells using cytokine secretion assay and flow cytometry (CSA-Flow).

5. Identification and Characterization of Antigen-Specific CD8⁺ T Cells Using Surface-Trapped TNF-α and Single-Cell Sequencing.