BACKGROUND: To assess the level of autofluorescence (lipofuscin) of atrophic parapapillary zones in different stages of glaucomatous optic disc atrophy. METHODS: Controlled cross-sectional prospective analysis of 79 consecutive eyes (15 normals as controls, 26 with ocular hypertension, 38 with primary open angle glaucoma). Eyes with retinal diseases or retinal pigment epithelial pathologies were excluded. The confocal scanning laser ophthalmoscope (HRA, Heidelberg Retina Angiograph) was used after lipofuscin excitation with argon blue laser (488 nm) to detect parapapillary autofluorescence in a spectrum above 500 nm. Size, extension of the parapapillary autofluorescent area and its mean distance to the optic nerve head were measured using the HRA standard software. Additional optic nerve head photographs taken with the 15 degrees Zeiss telecentric fundus camera (30 degrees camera with 2 x magnifier) were examined by two experienced ophthalmologists to determine the stage of glaucomatous optic disc atrophy (stages 0 to 4). RESULTS: Very small autofluorescent areas were found in vital discs (optic nerve glaucoma stage 0) in the parapapillary atrophic area (0.08 +/- 0.12 mm (2)) in contrast to glaucomatous discs in stage 1 (0.24 +/- 0.26 mm (2)) and stages 2, 3 and 4 (0.59 +/- 1.29 mm (2), logistic regression analysis r = 0.71; P = 0.029). The circular extension of the autofluorescent area correlated borderlined with the stage of the glaucomatous disc atrophy (higher glaucoma stages: r = 0.82; P = 0.09). The autofluorescent area was larger in OHT than in controls (0.11 mm (2) vs. 0.04 mm (2), P < 0.03). The circular extension of the autofluorescent area was longer in OHT than in controls (0.5 mm vs. 1.15 mm, P < 0.04). CONCLUSIONS: As a sign of pronounced lipofuscin accumulation in the parapapillary atrophic zone higher degrees of fundus autofluorescence can be detected in OHT and manifest primary open angle glaucoma in contrast to normals. The lipofuscin accumulation is correlated with the stage of progression of glaucoma and the stage of optic disc atrophy. The detection of active parapapillary autofluorescent areas especially in OHT may offer the ophthalmologist an important tool for early diagnosis.
BACKGROUND: To assess the level of autofluorescence (lipofuscin) of atrophic parapapillary zones in different stages of glaucomatous optic disc atrophy. METHODS: Controlled cross-sectional prospective analysis of 79 consecutive eyes (15 normals as controls, 26 with ocular hypertension, 38 with primary open angle glaucoma). Eyes with retinal diseases or retinal pigment epithelial pathologies were excluded. The confocal scanning laser ophthalmoscope (HRA, Heidelberg Retina Angiograph) was used after lipofuscin excitation with argon blue laser (488 nm) to detect parapapillary autofluorescence in a spectrum above 500 nm. Size, extension of the parapapillary autofluorescent area and its mean distance to the optic nerve head were measured using the HRA standard software. Additional optic nerve head photographs taken with the 15 degrees Zeiss telecentric fundus camera (30 degrees camera with 2 x magnifier) were examined by two experienced ophthalmologists to determine the stage of glaucomatous optic disc atrophy (stages 0 to 4). RESULTS: Very small autofluorescent areas were found in vital discs (optic nerve glaucoma stage 0) in the parapapillary atrophic area (0.08 +/- 0.12 mm (2)) in contrast to glaucomatous discs in stage 1 (0.24 +/- 0.26 mm (2)) and stages 2, 3 and 4 (0.59 +/- 1.29 mm (2), logistic regression analysis r = 0.71; P = 0.029). The circular extension of the autofluorescent area correlated borderlined with the stage of the glaucomatous disc atrophy (higher glaucoma stages: r = 0.82; P = 0.09). The autofluorescent area was larger in OHT than in controls (0.11 mm (2) vs. 0.04 mm (2), P < 0.03). The circular extension of the autofluorescent area was longer in OHT than in controls (0.5 mm vs. 1.15 mm, P < 0.04). CONCLUSIONS: As a sign of pronounced lipofuscin accumulation in the parapapillary atrophic zone higher degrees of fundus autofluorescence can be detected in OHT and manifest primary open angle glaucoma in contrast to normals. The lipofuscin accumulation is correlated with the stage of progression of glaucoma and the stage of optic disc atrophy. The detection of active parapapillary autofluorescent areas especially in OHT may offer the ophthalmologist an important tool for early diagnosis.
Authors: Robert Laemmer; Folkert K Horn; Arne Viestenz; Barbara Link; Anselm G Juenemann; Christian Y Mardin Journal: Graefes Arch Clin Exp Ophthalmol Date: 2006-08-01 Impact factor: 3.117
Authors: Kaweh Mansouri; Jonathan D Tung; Felipe A Medeiros; Ali Tafreshi; Syril Dorairaj; Linda Zangwill; Feng He; Sonia Jain; Robert N Weinreb Journal: Acta Ophthalmol Date: 2013-03-18 Impact factor: 3.761