Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification and characterization of a DNA-dependent ATPase from Escherichia coli.

Literature DB >> 129472

Purification and characterization of a DNA-dependent ATPase from Escherichia coli.

Abstract

A DNA-dependent ATPase has been isolated and purified from an Escherichia coli cell-free extract. The ATPase has the following characteristics: preferential dependence on single-stranded DNA, specificity for ATP hydrolysis, Km value of 1.4 X 10-4 M for ATP, and molecular weight of approximately 69,000. The ATPase can be shown to bind to single stranded DNA. The resemblance between this ATPase and that isolated from vaccinia cores is discussed.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1976 PMID： 129472

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

Keyword Cloud
Cited

16 in total

1. Enzyme-catalyzed DNA unwinding: studies on Escherichia coli rep protein.

Authors: G T Yarranton; M L Gefter
Journal: Proc Natl Acad Sci U S A Date: 1979-04 Impact factor: 11.205

2. UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.

Authors: Jae Young Lee; Wei Yang
Journal: Cell Date: 2006-12-29 Impact factor: 41.582

3. Conserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity.

Authors: G Zhang; E Deng; L R Baugh; C M Hamilton; V F Maples; S R Kushner
Journal: J Bacteriol Date: 1997-12 Impact factor: 3.490

4. Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency.

Authors: G Zhang; E Deng; L Baugh; S R Kushner
Journal: J Bacteriol Date: 1998-01 Impact factor: 3.490

5. Prophage induction and cell division in E. coli. V. Dominance and complementation analysis in partial diploids with pleiotropic mutations (tif, recA, zab and lexB) at the recA locus.

Authors: M Castellazzi; P Morand; J George; G Buttin
Journal: Mol Gen Genet Date: 1977-06-24

10. Replication of M13 oriC bacteriophages in Escherichia coli rep mutant is dependent on the cloned Escherichia coli replication origin.

Authors: L S Kaguni; J M Kaguni; D S Ray
Journal: J Bacteriol Date: 1981-02 Impact factor: 3.490

Purification and characterization of a DNA-dependent ATPase from Escherichia coli.

1. Enzyme-catalyzed DNA unwinding: studies on Escherichia coli rep protein.

2. UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.

3. Conserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity.

4. Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency.

5. Prophage induction and cell division in E. coli. V. Dominance and complementation analysis in partial diploids with pleiotropic mutations (tif, recA, zab and lexB) at the recA locus.

6. Escherichia coli membrane proteins with an affinity for deoxyribonucleic acid.

7. Escherichia coli uvrD mutants with thermosensitive DNA-dependent adenosine triphosphatase I (helicase II).

8. The E. coli uvrD gene product is DNA helicase II.

9. Ecto-ATPase.

10. Replication of M13 oriC bacteriophages in Escherichia coli rep mutant is dependent on the cloned Escherichia coli replication origin.