Literature DB >> 129446

Prolonged methanol fixation of soluble mucosubstances in mucopolysaccharidoses.

M Elleder.   

Abstract

A simple and efficient method for the demonstration of highly water soluble acid mucosubstances in cold microtone sections is described. It consists of prolonged treatment of cold microtome sections with methanol (for at least 1 h) and subsequent staining with 0.1% azure A in distilled water or in 30% methanol. The procedure is recommended particularly for the bioptical examination of mucopolysaccharidoses.

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Year:  1976        PMID： 129446     DOI： 10.1007/bf02462740

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  7 in total

1.  HURLER'S SYNDROME: A HISTOCHEMICAL STUDY. NEW TECHNIQUES FOR LOCALIZATION OF VERY WATER-SOLUBLE ACID MUGOPOLYSACCHARIDES.

Authors:  H J WOLFE; J B BLENNERHASSET; G F YOUNG; R B COHEN
Journal:  Am J Pathol       Date:  1964-12       Impact factor: 4.307

2.  FIXATION IN HISTOCHEMISTRY.

Authors:  Z LOJDA
Journal:  Folia Morphol (Praha)       Date:  1965

3.  Histochemical studies in Hurler's disease: a new method for localization of acid mucopolysaccharide, and an analysis of lead acetate "fixation".

Authors:  M D HAUST; B H LANDING
Journal:  J Histochem Cytochem       Date:  1961-01       Impact factor: 2.479

4.  [Topochemistry of beta-glycosidases in the aortae and coronary arteries of rats, guinea pigs and rabbits under normal conditions and after cholesterol feed].

Authors:  Z Lojda
Journal:  Cesk Patol       Date:  1974-02

5.  The effect of various fixatives on the preservation of acid glycosaminoglycans in tissues.

Authors:  B Engfeldt; S O Hjertquist
Journal:  Acta Pathol Microbiol Scand       Date:  1967

6.  Ultrastructure and function of eccrine glands in the mucopolysaccharidoses.

Authors:  R W Belcher
Journal:  Arch Pathol       Date:  1973-11

7.  Further studies on metachromasia in cultured human fibroblasts. Staining of glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions.

Authors:  B S Danes; J E Scott; A G Bearn
Journal:  J Exp Med       Date:  1970-10-01       Impact factor: 14.307
  7 in total
  4 in total

1.  The cornea in normal condition and in Groenouw's macular dystrophy.

Authors:  J François; V Victoria-Troncoso
Journal:  Doc Ophthalmol       Date:  1979-11-30       Impact factor: 2.379

2.  Localisation of sulphated glycosaminoglycans in the mucopolysaccharidoses by a simple technique using cryostat sections.

Authors:  J Dorling
Journal:  J Clin Pathol       Date:  1980-09       Impact factor: 3.411

3.  Multiple sulphatase deficiency in homozygotic twins.

Authors:  S Nevsímalová; M Elleder; F Smíd; M Zemánková
Journal:  J Inherit Metab Dis       Date:  1984       Impact factor: 4.982

4.  Autoradiographic detection of mucopolysaccharide accumulation in single fibroblasts.

Authors:  P M Frederik; J J Fortuin; D Klepper; H Galjaard
Journal:  Histochem J       Date:  1977-01
  4 in total

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